Proportion of the primary and the secondary human graft
. | . | . | Bone marrow, % . | . | . | . | . | Thymus, % . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mouse . | Cell dose* . | Week† . | CD45 . | EGFP . | S . | M . | B . | CD45 . | EGFP . | T . | ||||||
| Primary recipient | ||||||||||||||||
| 101 | 17 | 13 | 80.3 | 72.7 | ND | ND | ND | ND | ND | ND | ||||||
| 109 | 6 | 19 | 66.2 | 71.0 | ND | ND | ND | ND | ND | ND | ||||||
| 113 | 10 | 14 | 43.2 | 71.8 | ND | ND | ND | ND | ND | ND | ||||||
| Secondary recipient | ||||||||||||||||
| 101-1 | 21 | 13 | 63.0 | 93.3 | 3.4 | 7.4 | 63.7 | 98.2 | 80.1 | 83.6 | ||||||
| 101-2 | 21 | 13 | 62.5 | 88.1 | 4.2 | 14.6 | 54.9 | 96.5 | 78.6 | 71.2 | ||||||
| 109-1 | 13.5 | 17 | 29.4 | 69.4 | 1.3 | 49.5 | 29.9 | 91.3 | 78.2 | 90.5 | ||||||
| 109-2 | 13.5 | 17 | 33.3 | 75.1 | 1.9 | 27.6 | 45.9 | 93.0 | 70.1 | 81.1 | ||||||
| 113-1 | 19.3 | 19 | 72.3 | 68.7 | 3.4 | 17.3 | 38.7 | 95.5 | 88.1 | 88.0 | ||||||
| 113-2 | 19.3 | 19 | 86.3 | 50.0 | 0.6 | 17.2 | 17.3 | 92.9 | 72.3 | 70.3 | ||||||
. | . | . | Bone marrow, % . | . | . | . | . | Thymus, % . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mouse . | Cell dose* . | Week† . | CD45 . | EGFP . | S . | M . | B . | CD45 . | EGFP . | T . | ||||||
| Primary recipient | ||||||||||||||||
| 101 | 17 | 13 | 80.3 | 72.7 | ND | ND | ND | ND | ND | ND | ||||||
| 109 | 6 | 19 | 66.2 | 71.0 | ND | ND | ND | ND | ND | ND | ||||||
| 113 | 10 | 14 | 43.2 | 71.8 | ND | ND | ND | ND | ND | ND | ||||||
| Secondary recipient | ||||||||||||||||
| 101-1 | 21 | 13 | 63.0 | 93.3 | 3.4 | 7.4 | 63.7 | 98.2 | 80.1 | 83.6 | ||||||
| 101-2 | 21 | 13 | 62.5 | 88.1 | 4.2 | 14.6 | 54.9 | 96.5 | 78.6 | 71.2 | ||||||
| 109-1 | 13.5 | 17 | 29.4 | 69.4 | 1.3 | 49.5 | 29.9 | 91.3 | 78.2 | 90.5 | ||||||
| 109-2 | 13.5 | 17 | 33.3 | 75.1 | 1.9 | 27.6 | 45.9 | 93.0 | 70.1 | 81.1 | ||||||
| 113-1 | 19.3 | 19 | 72.3 | 68.7 | 3.4 | 17.3 | 38.7 | 95.5 | 88.1 | 88.0 | ||||||
| 113-2 | 19.3 | 19 | 86.3 | 50.0 | 0.6 | 17.2 | 17.3 | 92.9 | 72.3 | 70.3 | ||||||
The total cellularity of BM in the primary and the secondary recipient was 3.86 × 107 ± 0.83 × 107 and 3.72 × 107 ± 0.91 × 107, respectively. The total cellularity of thymus in the primary and the secondary recipient was 3.36 × 105 ± 3.28 × 105 and 2.43 × 105 ± 1.59 × 105, respectively.
ND indicates not done.
Number of CD34+ CD38- cells transplanted (primary recipient, × 103; secondary recipient, × 106).
Number of weeks after transplantation.