Specific primers for amplification of ADAMTS13 in human endothelial cells
Domains/primers . | Sequences . | Size, bp . |
|---|---|---|
| Signal peptide | ||
| 128F | 5′-cac agg ccg tgt ctt ctt act tga-3′ | 286 |
| 414R | 5′-tca ggc tct gtc aga atg acc atc-3′ | |
| Metalloprotease | ||
| 390F | 5′-gat ggt cat tct gac aga gcc tga-3′ | 178 |
| 568R | 5′-gtt acc atc agg caa ctc cag-3′ | |
| Spacer | ||
| 1691F | 5′-ctg gca gag cga gag aat atg tca-3′ | 197 |
| 1888R | 5′-tct gta ctc gac acg acc atc ctc-3′ | |
| Second CUB | ||
| 3991F | 5′-gtg gct ccg cac gca cgg att-3′ | 291 |
| 4282R | 5′-ggt tcc ttc ctt tcc ctt cca gg-3 |
Domains/primers . | Sequences . | Size, bp . |
|---|---|---|
| Signal peptide | ||
| 128F | 5′-cac agg ccg tgt ctt ctt act tga-3′ | 286 |
| 414R | 5′-tca ggc tct gtc aga atg acc atc-3′ | |
| Metalloprotease | ||
| 390F | 5′-gat ggt cat tct gac aga gcc tga-3′ | 178 |
| 568R | 5′-gtt acc atc agg caa ctc cag-3′ | |
| Spacer | ||
| 1691F | 5′-ctg gca gag cga gag aat atg tca-3′ | 197 |
| 1888R | 5′-tct gta ctc gac acg acc atc ctc-3′ | |
| Second CUB | ||
| 3991F | 5′-gtg gct ccg cac gca cgg att-3′ | 291 |
| 4282R | 5′-ggt tcc ttc ctt tcc ctt cca gg-3 |