Percentage of JAK2V617F mutation in the cDNA of CD34+ progenitor cells and their differentiated cellular progenies generated by SCF, IL-6, IL-3, and various dose of Epo
. | . | Following 12 days of culture with SCF ± IL-6 ± IL-3 plus different concentrations of Epo . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Donors* . | CD34+ cells before culture, % . | 0 U/mL, % . | 0.025 U/mL, % . | 0.1 U/mL, % . | 0.5 U/mL, % . | 4 U/mL, % . | ||||
| PV2 | 34.6 | 32.4 | 29.5 | 17.0 | 4.9 | 5.1 | ||||
| PV4 | 71.0 | 49.3 | 35.6 | 19.1 | 9.8 | 12.1 | ||||
| PV7 | 71.0 | 65.9 | 40.1 | 18.9 | 10.7 | 9.8 | ||||
| PV8 | 76.6 | 71.4 | 70.1 | 57.9 | 46.4 | 53.1 | ||||
. | . | Following 12 days of culture with SCF ± IL-6 ± IL-3 plus different concentrations of Epo . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Donors* . | CD34+ cells before culture, % . | 0 U/mL, % . | 0.025 U/mL, % . | 0.1 U/mL, % . | 0.5 U/mL, % . | 4 U/mL, % . | ||||
| PV2 | 34.6 | 32.4 | 29.5 | 17.0 | 4.9 | 5.1 | ||||
| PV4 | 71.0 | 49.3 | 35.6 | 19.1 | 9.8 | 12.1 | ||||
| PV7 | 71.0 | 65.9 | 40.1 | 18.9 | 10.7 | 9.8 | ||||
| PV8 | 76.6 | 71.4 | 70.1 | 57.9 | 46.4 | 53.1 | ||||
The purity of the CD34+ cells was ≥95%. The CD34+ cells were cultured in the presence of SCF, IL-3, IL-6 and different concentrations of Epo for 12 days. Each value represents the JAK2V617F mutant allele/JAK2 total allele × 100 in the cDNA. JAK2V617F/JAK2total was calculated according to the mean ΔCt and the standard curve by real time quantitative PCR.
The G-CSF—mobilized healthy subject had 0% JAK2V617F mutation.