Percentage of JAK2V617F mutation in the cDNA of CD34+ progenitor cells and their differentiated cellular progenies
. | . | Following culture . | . | |
|---|---|---|---|---|
| Donors* . | CD34+ cells before culture, % . | MNCs at day 6, % . | MNCs at day 12, % . | |
| PV1 | 85.0 | 73.7 | 66.9 | |
| PV2 | 34.6 | 10.5 | 5.1 | |
| PV3 | 98.7 | 85.7 | 77.9 | |
| PV4 | 71.0 | 28.3 | 12.1 | |
| PV7 | 71.0 | 39.9 | 9.8 | |
| PV8 | 76.6 | 74.7 | 53.1 | |
| PV9 | 100 | 100 | 100 | |
| PV10 | 83.9 | 70.8 | 43.5 | |
. | . | Following culture . | . | |
|---|---|---|---|---|
| Donors* . | CD34+ cells before culture, % . | MNCs at day 6, % . | MNCs at day 12, % . | |
| PV1 | 85.0 | 73.7 | 66.9 | |
| PV2 | 34.6 | 10.5 | 5.1 | |
| PV3 | 98.7 | 85.7 | 77.9 | |
| PV4 | 71.0 | 28.3 | 12.1 | |
| PV7 | 71.0 | 39.9 | 9.8 | |
| PV8 | 76.6 | 74.7 | 53.1 | |
| PV9 | 100 | 100 | 100 | |
| PV10 | 83.9 | 70.8 | 43.5 | |
The purity of the CD34+ cells was 95% or greater. The CD34+ cells were cultured in the presence of SCF, IL-3, IL-6, and Epo at their optimal concentration for 6 and 12 days. Each value represents the JAK2V617F mutant allele/JAK2 total allele × 100 in the cDNA. JAK2V617F/JAK2total was calculated according to the mean ΔCt and the standard curve by real-time quantitative PCR.
G-CSF—mobilized healthy subjects 1 and 2 had 0% JAK2V617F mutation.