Morphologic analysis of PB CD34+ cells isolated from patients with PV and healthy G-CSF–mobilized donors cultured in vitro for 6 days and 12 days
. | Myeloblast, % . | Promyeloblast, % . | Myelocyte, % . | Metamyelocyte, % . | Normoblasts, % . |
|---|---|---|---|---|---|
| PV | |||||
| Day 6 | 14.6 ± 4.1 | 61.3 ± 6.2 | 11.2 ± 8.2 | 2.3 ± 1.5 | 4.9 ± 4.1 |
| Day 12 | 2.1 ± 1.7 | 31.0 ± 6.5 | 23.2 ± 10.0 | 18.4 ± 6.3 | 7.6 ± 4.3 |
| Mob | |||||
| Day 6 | 10.9 ± 2.5 | 49.1 ± 5.1 | 35.5 ± 7.7 | 2.0 ± 1.0 | 2.5 ± 3.0 |
| Day 12 | 2.5 ± 1.4 | 26.5 ± 4.9 | 41.3 ± 12.2 | 20.0 ± 6.8 | 9.7 ± 4.6 |
. | Myeloblast, % . | Promyeloblast, % . | Myelocyte, % . | Metamyelocyte, % . | Normoblasts, % . |
|---|---|---|---|---|---|
| PV | |||||
| Day 6 | 14.6 ± 4.1 | 61.3 ± 6.2 | 11.2 ± 8.2 | 2.3 ± 1.5 | 4.9 ± 4.1 |
| Day 12 | 2.1 ± 1.7 | 31.0 ± 6.5 | 23.2 ± 10.0 | 18.4 ± 6.3 | 7.6 ± 4.3 |
| Mob | |||||
| Day 6 | 10.9 ± 2.5 | 49.1 ± 5.1 | 35.5 ± 7.7 | 2.0 ± 1.0 | 2.5 ± 3.0 |
| Day 12 | 2.5 ± 1.4 | 26.5 ± 4.9 | 41.3 ± 12.2 | 20.0 ± 6.8 | 9.7 ± 4.6 |
The purity of the CD34+ cells was 95% or greater. The CD34+ cells were cultured in the presence of SCF, IL-3, IL-6, and Epo at their optimal concentration for 12 days. Cytospin preparations of the cells (day 6 and day 12) were prepared and stained with May-Grünwald-Giemsa. A 100- to 200-cell differential was performed under × 400 magnification using an Olympus BH-2 light microscope. Data are presented as mean ± SD.
Mob indicates G-CSF—mobilized healthy subject.