Table 1. Detection of the JAK2 V617F... | American Society of Hematology

Table 1.

Detection of the JAK2^V617F mutation in gDNA of various hematopoietic lineages isolated from the PB of patients with PV and healthy volunteers by direct sequencing

Donors*	Granulocytes	CD34⁺ cells	CD33⁺ cells	Gly-A⁺ cells	CD19⁺ cells	CD3⁺ cells
PV1	+	+	+	+	+	-
PV2	+	+	+	+	-	-
PV3	+	+	+	+	-	-
PV4	+	+	+	+	-	-
PV7	+	+	+	+	-	-
PV8	+	+	+	+	-	-
PV9	++	++	++	++	+	+
PV10	+	+	+	+	-	-

Donors*	Granulocytes	CD34⁺ cells	CD33⁺ cells	Gly-A⁺ cells	CD19⁺ cells	CD3⁺ cells
PV1	+	+	+	+	+	-
PV2	+	+	+	+	-	-
PV3	+	+	+	+	-	-
PV4	+	+	+	+	-	-
PV7	+	+	+	+	-	-
PV8	+	+	+	+	-	-
PV9	++	++	++	++	+	+
PV10	+	+	+	+	-	-

The purity of the CD19⁺ cells and CD3⁺ cells was 99% or greater. The purity of the other cell types was 95% or greater.

- indicates no detectable G-T substitution in the sequence; +, heterozygous G-T substitution; ++, homozygous G-T substitution; Mob, G-CSF mobilized healthy subject.

Mob1, Mob2, and Mob3 had no detectable G-T substitution in the sequence.

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