Evaluation of endothelial cell surface integrin expression
. | Median intensity of fluorescence, arbitry unit, log scale . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| Cell type . | Control . | Integrin αvβ3 . | Integrin αvβ5 . | Integrin α5β1 . | |||
| HUVECs on VN | 1.1 | 28.9 | 8.4 | 67 | |||
| HUVECs on FN | 1.2 | 31.3 | 8.8 | 62.1 | |||
| CDC.HMEC-1 on VN | 1.3 | 28.4 | 7.4 | 72.4 | |||
| CDC.HMEC-1 on FN | 2 | 25.6 | 9.2 | 69.3 | |||
. | Median intensity of fluorescence, arbitry unit, log scale . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| Cell type . | Control . | Integrin αvβ3 . | Integrin αvβ5 . | Integrin α5β1 . | |||
| HUVECs on VN | 1.1 | 28.9 | 8.4 | 67 | |||
| HUVECs on FN | 1.2 | 31.3 | 8.8 | 62.1 | |||
| CDC.HMEC-1 on VN | 1.3 | 28.4 | 7.4 | 72.4 | |||
| CDC.HMEC-1 on FN | 2 | 25.6 | 9.2 | 69.3 | |||
HUVECs and CDC.HMEC-1 cells were grown for 3 hours in experimental medium on VN- or FN-coated plates. Cell-surface integrin expression was measured by flow cytometry. Results of 1 of 2 experiments with similar findings are shown.