Characteristics of HMCLs classified according to HGS-ETR1 sensitivity
. | . | . | . | Death induced by HGS-ETR1 (μg/mL), % . | . | . | Death induced by HGS-ETR2 (μg/mL), % . | . | ||
|---|---|---|---|---|---|---|---|---|---|---|
. | 14q32 locus . | IL-6 . | DR4, ratio . | 0.6 . | 6 . | DR5, ratio . | 0.6 . | 6 . | ||
| HGS-ETR1 resistant | ||||||||||
| ANBL-6 | t(14,16) | + | 1.4 | 1 ± 1 | 1 ± 1 | 1 | 1 ± 1 | 1 ± 1 | ||
| BCN | t(14,16) | + | 2.7 | 1 ± 1 | 1 ± 1 | 2.8 | 1 ± 1 | 1 ± 1 | ||
| JJN3 | t(14,16) | - | 1.3 | 1 ± 1 | 1 ± 1 | 1.2 | 1 ± 1 | 1 ± 1 | ||
| NAN5 | t(4,14) | + | 1.2 | 0 ± 1 | 2 ± 1 | 1 | 1 ± 1 | 2 ± 1 | ||
| SBN | GL | + | 4.3 | 0 ± 0 | 0 ± 0 | 1.8 | 12 ± 9 | 18 ± 1 | ||
| XG6 | GL | + | 3.2 | 4 ± 1 | 5 ± 4 | 1.9 | 3 ± 3 | 10 ± 13 | ||
| XG7 | t(6,14) | + | 1.8 | 3 ± 2 | 7 ± 7 | 1.9 | 0 ± 0 | 11 ± 15 | ||
| HGS-ETR1 sensitive | ||||||||||
| NCI-H929 | t(4,14) | - | 2.8 | 11 ± 7 | 17 ± 8 | 2.4 | 26 ± 4 | 53 ± 8 | ||
| KMS12BM | t(11,14) | - | 1.5 | 5 ± 4 | 32 ± 10 | 2.1 | 1 ± 1 | 5 ± 1 | ||
| L363 | GL | - | 4.4 | 23 ± 11 | 31 ± 10 | 1.9 | 7 ± 3 | 11 ± 5 | ||
| KMS18 | t(4,14) | - | 1.4 | 19 ± 3 | 39 ± 6 | 2.9 | 8 ± 4 | 16 ± 3 | ||
| U266 | t(11,14) | + | 3.2 | 28 ± 5 | 35 ± 15 | 1.1 | 1 ± 1 | 1 ± 1 | ||
| LP1 | t(4,14) | - | 5.4 | 31 ± 18 | 41 ± 4 | 2.3 | 1 ± 1 | 1 ± 1 | ||
| NAN1 | t(14,16) | + | 2.2 | 29 ± 16 | 41 ± 20 | 2.2 | 1 ± 1 | 1 ± 1 | ||
| NAN3 | t(4,14) | + | 3.5 | 32 ± 0 | 44 ± 5 | 1.5 | 1 ± 1 | 1 ± 1 | ||
| XG1 | t(11,14) | + | 2.7 | 47 ± 14 | 51 ± 15 | 1.9 | 9 ± 6 | 3 ± 6 | ||
| NAN4 | GL | + | 3.6 | 41 ± 13 | 58 ± 14 | 1.1 | 1 ± 2 | 1 ± 1 | ||
| RPMI8226 | t(14,16) | - | 3.1 | 51 ± 18 | 61 ± 3 | 3.8 | 4 ± 1 | 10 ± 12 | ||
| XG2 | t(12,14) | + | 3.2 | 38 ± 20 | 62 ± 3 | 1.3 | 1 ± 1 | 1 ± 1 | ||
| XG5 | t(11,14) | + | 3.5 | 43 ± 1 | 68 ± 1 | 4.9 | 9 ± 4 | 18 ± 8 | ||
| KMS12PE | t(11,14) | - | 2.5 | 42 ± 7 | 77 ± 11 | 3.8 | 3 ± 1 | 14 ± 6 | ||
| MDN | t(11,14) | + | 3.7 | 79 ± 12 | 79 ± 7 | 1.8 | 50 ± 1 | 61 ± 21 | ||
. | . | . | . | Death induced by HGS-ETR1 (μg/mL), % . | . | . | Death induced by HGS-ETR2 (μg/mL), % . | . | ||
|---|---|---|---|---|---|---|---|---|---|---|
. | 14q32 locus . | IL-6 . | DR4, ratio . | 0.6 . | 6 . | DR5, ratio . | 0.6 . | 6 . | ||
| HGS-ETR1 resistant | ||||||||||
| ANBL-6 | t(14,16) | + | 1.4 | 1 ± 1 | 1 ± 1 | 1 | 1 ± 1 | 1 ± 1 | ||
| BCN | t(14,16) | + | 2.7 | 1 ± 1 | 1 ± 1 | 2.8 | 1 ± 1 | 1 ± 1 | ||
| JJN3 | t(14,16) | - | 1.3 | 1 ± 1 | 1 ± 1 | 1.2 | 1 ± 1 | 1 ± 1 | ||
| NAN5 | t(4,14) | + | 1.2 | 0 ± 1 | 2 ± 1 | 1 | 1 ± 1 | 2 ± 1 | ||
| SBN | GL | + | 4.3 | 0 ± 0 | 0 ± 0 | 1.8 | 12 ± 9 | 18 ± 1 | ||
| XG6 | GL | + | 3.2 | 4 ± 1 | 5 ± 4 | 1.9 | 3 ± 3 | 10 ± 13 | ||
| XG7 | t(6,14) | + | 1.8 | 3 ± 2 | 7 ± 7 | 1.9 | 0 ± 0 | 11 ± 15 | ||
| HGS-ETR1 sensitive | ||||||||||
| NCI-H929 | t(4,14) | - | 2.8 | 11 ± 7 | 17 ± 8 | 2.4 | 26 ± 4 | 53 ± 8 | ||
| KMS12BM | t(11,14) | - | 1.5 | 5 ± 4 | 32 ± 10 | 2.1 | 1 ± 1 | 5 ± 1 | ||
| L363 | GL | - | 4.4 | 23 ± 11 | 31 ± 10 | 1.9 | 7 ± 3 | 11 ± 5 | ||
| KMS18 | t(4,14) | - | 1.4 | 19 ± 3 | 39 ± 6 | 2.9 | 8 ± 4 | 16 ± 3 | ||
| U266 | t(11,14) | + | 3.2 | 28 ± 5 | 35 ± 15 | 1.1 | 1 ± 1 | 1 ± 1 | ||
| LP1 | t(4,14) | - | 5.4 | 31 ± 18 | 41 ± 4 | 2.3 | 1 ± 1 | 1 ± 1 | ||
| NAN1 | t(14,16) | + | 2.2 | 29 ± 16 | 41 ± 20 | 2.2 | 1 ± 1 | 1 ± 1 | ||
| NAN3 | t(4,14) | + | 3.5 | 32 ± 0 | 44 ± 5 | 1.5 | 1 ± 1 | 1 ± 1 | ||
| XG1 | t(11,14) | + | 2.7 | 47 ± 14 | 51 ± 15 | 1.9 | 9 ± 6 | 3 ± 6 | ||
| NAN4 | GL | + | 3.6 | 41 ± 13 | 58 ± 14 | 1.1 | 1 ± 2 | 1 ± 1 | ||
| RPMI8226 | t(14,16) | - | 3.1 | 51 ± 18 | 61 ± 3 | 3.8 | 4 ± 1 | 10 ± 12 | ||
| XG2 | t(12,14) | + | 3.2 | 38 ± 20 | 62 ± 3 | 1.3 | 1 ± 1 | 1 ± 1 | ||
| XG5 | t(11,14) | + | 3.5 | 43 ± 1 | 68 ± 1 | 4.9 | 9 ± 4 | 18 ± 8 | ||
| KMS12PE | t(11,14) | - | 2.5 | 42 ± 7 | 77 ± 11 | 3.8 | 3 ± 1 | 14 ± 6 | ||
| MDN | t(11,14) | + | 3.7 | 79 ± 12 | 79 ± 7 | 1.8 | 50 ± 1 | 61 ± 21 | ||
TRAIL-R1 (DR4) or TRAIL-R2 (DR5) expression was measured by FACS and expressed as the ratio of fluorescence (specific fluorescence divided by isotype control fluorescence). The percentage of specific cell death was measured after 48-hour incubation with 0.6 or 6 μg/mL HGS-ETR1 or HGS-ETR2 mAb. HMCLs cultured with IL-6 are indicated. Values of death are mean ± SD of at least 3 independent experiments.