Secondary transplantation of BM cells reconstituted with single SP- or MP-CD34-KSL cells
. | Primary Tx . | Secondary Tx . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| Cell type . | Total . | Total . | Myeloid . | B-lymphoid . | T-lymphoid . | |||
| SP | ||||||||
| Recipient 1 | 66.4 | 45.2 ± 20.2 | 3.1 ± 2.6 | 84.2 ± 14.9 | 82.9 ± 16.3 | |||
| Recipient 2 | 6.3 | 0.3 ± 0.3 | 0.2 ± 0.5 | 0.3 ± 0.3 | 0.2 ± 0.2 | |||
| MP | ||||||||
| Recipient 1 | 53.0 | 63.8 ± 18.3 | 58.6 ± 26.8 | 63.0 ± 17.2 | 83.6 ± 9.0 | |||
| Recipient 2 | 23.6 | 3.3 ± 3.6 | 0.9 ± 0.8 | 4.6 ± 2.3 | 10.5 ± 19.4 | |||
. | Primary Tx . | Secondary Tx . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| Cell type . | Total . | Total . | Myeloid . | B-lymphoid . | T-lymphoid . | |||
| SP | ||||||||
| Recipient 1 | 66.4 | 45.2 ± 20.2 | 3.1 ± 2.6 | 84.2 ± 14.9 | 82.9 ± 16.3 | |||
| Recipient 2 | 6.3 | 0.3 ± 0.3 | 0.2 ± 0.5 | 0.3 ± 0.3 | 0.2 ± 0.2 | |||
| MP | ||||||||
| Recipient 1 | 53.0 | 63.8 ± 18.3 | 58.6 ± 26.8 | 63.0 ± 17.2 | 83.6 ± 9.0 | |||
| Recipient 2 | 23.6 | 3.3 ± 3.6 | 0.9 ± 0.8 | 4.6 ± 2.3 | 10.5 ± 19.4 | |||
Six months after primary transplantation, 2 × 106 BM cells from 2 recipient mice reconstituted with single SP-CD34-KSL cells and from 2 recipient mice reconstituted with single MP-CD34-KSL cells (Table 3) were transplanted into lethally irradiated mice. Secondary recipient mice were analyzed 4 months after transplantation. The percent chimerism is shown as mean ± SD for total leukocytes, myeloid cells, B-lymphoid cells, and T-lymphoid cells. For the secondary recipients of SP cells, n = 7 (recipient 1) and n = 9 (recipient 2); for the secondary recipients of MP cells, n = 9 (recipient 1) and n = 7 (recipient 2)
Tx indicates transplantation