Properties of liver granulomata of S mansoni–infected mice
Wk of infection/mouse strain . | Granulomata* . | Volume, mm3 × 10-3 . | Hydroxyproline, μM/g . | Hydroxyproline, μM/104 eggs . | Eosinophils, % total cells . | Mast cells, no. per granuloma . |
|---|---|---|---|---|---|---|
| 8 | ||||||
| BALB/c | nd | 28.6 ± 2.5 | nd | nd | 10.1 ± 2.3 | 7.2 ± 1.2 |
| ΔdblGATA | nd | 26.2 ± 1.5 | nd | nd | 0† | 8.5 ± 2.0 |
| 12 | ||||||
| BALB/c | 150 ± 12 | 27.4 ± 2.2 | 16.6 ± 0.9 | 2.5 ± 1.4 | 33.2 ± 4 | 3.1 ± 0.5 |
| ΔdblGATA | 130 ± 8 | 27.6 ± 3.2 | 16.3 ± 1.1 | 4.7 ± 0.7 | 0† | 5.5 ± 0.4† |
| C57BL/6 | 228 ± 16 | 20.1 ± 1.4 | 10.6 ± 0.6 | 2.4 ± 1.2 | 51.1 ± 2.9 | 1.1 ± 0.3 |
| TgPHIL | 219 ± 20 | 16.7 ± 1.5 | 11.4 ± 0.5 | 3.5 ± 1.0 | 0†‡ | 2 ± 0.4 |
Wk of infection/mouse strain . | Granulomata* . | Volume, mm3 × 10-3 . | Hydroxyproline, μM/g . | Hydroxyproline, μM/104 eggs . | Eosinophils, % total cells . | Mast cells, no. per granuloma . |
|---|---|---|---|---|---|---|
| 8 | ||||||
| BALB/c | nd | 28.6 ± 2.5 | nd | nd | 10.1 ± 2.3 | 7.2 ± 1.2 |
| ΔdblGATA | nd | 26.2 ± 1.5 | nd | nd | 0† | 8.5 ± 2.0 |
| 12 | ||||||
| BALB/c | 150 ± 12 | 27.4 ± 2.2 | 16.6 ± 0.9 | 2.5 ± 1.4 | 33.2 ± 4 | 3.1 ± 0.5 |
| ΔdblGATA | 130 ± 8 | 27.6 ± 3.2 | 16.3 ± 1.1 | 4.7 ± 0.7 | 0† | 5.5 ± 0.4† |
| C57BL/6 | 228 ± 16 | 20.1 ± 1.4 | 10.6 ± 0.6 | 2.4 ± 1.2 | 51.1 ± 2.9 | 1.1 ± 0.3 |
| TgPHIL | 219 ± 20 | 16.7 ± 1.5 | 11.4 ± 0.5 | 3.5 ± 1.0 | 0†‡ | 2 ± 0.4 |
Analysis based on measurements from more than 100 granulomata (n = 8-13 mice per group) from Giemsa-stained liver tissue; methodology was as described.28,29 Data are presented as plus or minus standard error of the mean.
nd indicates not determined.
Number of single-egg granulomata was scored per 2.25 cm2 of infected liver tissue.
Significant differences between BALB/c and ΔdblGATA at given time point.
Five eosinophils were identified in one granuloma of one TgPHIL mouse.