Effect of signal molecules on ρ expression
Factor . | Induction (%) . | Inhibition, no. beads with effect/no. beads tested (%) . |
|---|---|---|
| BMP2 | 0/13 (0) | 0/9 (0) |
| BMP7 | 0/16 (0) | 0/12 (0) |
| Chordin | 0/37 (0) | 1/12 (8) |
| Noggin | 0/28 (0) | 0/20 (0) |
| FGF4 | 0/62 (0) | 40/53 (75) |
| FGF8 | 0/40 (0) | 15/29 (52) |
| Nodal | 0/24 (0) | 0/18 (0) |
| Dkk1 | 0/29 (0) | 0/22 (0) |
| VEGF165 | 0/27 (0) | 0/15 (0) |
| Wnt3A | 1/52 (2) | 0/28 (0) |
| Epo | 0/6 (0) | 0/4 (0) |
Factor . | Induction (%) . | Inhibition, no. beads with effect/no. beads tested (%) . |
|---|---|---|
| BMP2 | 0/13 (0) | 0/9 (0) |
| BMP7 | 0/16 (0) | 0/12 (0) |
| Chordin | 0/37 (0) | 1/12 (8) |
| Noggin | 0/28 (0) | 0/20 (0) |
| FGF4 | 0/62 (0) | 40/53 (75) |
| FGF8 | 0/40 (0) | 15/29 (52) |
| Nodal | 0/24 (0) | 0/18 (0) |
| Dkk1 | 0/29 (0) | 0/22 (0) |
| VEGF165 | 0/27 (0) | 0/15 (0) |
| Wnt3A | 1/52 (2) | 0/28 (0) |
| Epo | 0/6 (0) | 0/4 (0) |
Beads are grafted as indicated (Figure 3A). The effect of each factor is assayed by its ability to either induce (in ectopic or endogenous domain) or inhibit (endogenous) ρ expression in adjacent cells.