Table 1.

FACS determination of BrdU incorporation by Ter119+ E15.5 FL cells following 1 hour in vivo BrdU pulse


Genotype

n

Gate I, BrdU-, %2n*

Gate II, BrdU+, %2n to 4n*

Gate III, BrdU-, %above 2n to 4n*
E2f4+/+  7   53.6 ± 11.2   45.0 ± 11.7   1.0 ± 0.5  
E2f4+/-  12   49.9 ± 12.3   48.5 ± 12.1   1.3 ± 0.8  
E2f4-/-
 
8
 
38.4 ± 3.7
 
52.5 ± 3.7
 
8.4 ± 2.1
 

Genotype

n

Gate I, BrdU-, %2n*

Gate II, BrdU+, %2n to 4n*

Gate III, BrdU-, %above 2n to 4n*
E2f4+/+  7   53.6 ± 11.2   45.0 ± 11.7   1.0 ± 0.5  
E2f4+/-  12   49.9 ± 12.3   48.5 ± 12.1   1.3 ± 0.8  
E2f4-/-
 
8
 
38.4 ± 3.7
 
52.5 ± 3.7
 
8.4 ± 2.1
 
*

Gates correspond to gates in Figure 3B.

DNA content where 2n is the DNA content of a diploid gene.

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