Correction of hematologic pathology and urine concentration defect in mice by homologous recombination
Mice . | RBC, × 106/μL . | Hb, g/dL . | PCV, % . | Reticulocytes, % . | RDW, % . | MCV, fL . | MCH, pg . | Urine concentration, mOsm . |
|---|---|---|---|---|---|---|---|---|
| hβA/hβA control | 10.7 ± 0.5 | 10.5 ± 0.6 | 41.1 ± 1.9 | 7.7 ± 2.9 | 21.7 ± 2.0 | 38.4 ± 2.0 | 9.8 ± 0.8 | 2129 ± 281 |
| hβS/hβS sickle | 6.7 ± 1.1 | 7.1 ± 0.9 | 35.1 ± 3.3 | 75.4 ± 3.9 | 32.5 ± 2.1 | 52.4 ± 3.5 | 10.6 ± 1.4 | 768 ± 102 |
| hβA/hβS corrected | 11.3 ± 1.0* | 10.8 ± 1.0* | 41.7 ± 2.5* | 8.4 ± 2.6* | 22.6 ± 1.2* | 36.9 ± 2.7* | 9.6 ± 1.4 | 2388 ± 397* |
Mice . | RBC, × 106/μL . | Hb, g/dL . | PCV, % . | Reticulocytes, % . | RDW, % . | MCV, fL . | MCH, pg . | Urine concentration, mOsm . |
|---|---|---|---|---|---|---|---|---|
| hβA/hβA control | 10.7 ± 0.5 | 10.5 ± 0.6 | 41.1 ± 1.9 | 7.7 ± 2.9 | 21.7 ± 2.0 | 38.4 ± 2.0 | 9.8 ± 0.8 | 2129 ± 281 |
| hβS/hβS sickle | 6.7 ± 1.1 | 7.1 ± 0.9 | 35.1 ± 3.3 | 75.4 ± 3.9 | 32.5 ± 2.1 | 52.4 ± 3.5 | 10.6 ± 1.4 | 768 ± 102 |
| hβA/hβS corrected | 11.3 ± 1.0* | 10.8 ± 1.0* | 41.7 ± 2.5* | 8.4 ± 2.6* | 22.6 ± 1.2* | 36.9 ± 2.7* | 9.6 ± 1.4 | 2388 ± 397* |
All animals were analyzed at 6 to 8 weeks after birth. Values represent the mean ± SD. Statistical significance was determined for hβA/hβS-corrected mice compared with hβS/hβS sickle animals; P values were calculated using a 2-tailed Student t test; n ≥ 6.
P ≤ .001.