Active β-major transcription in orthochromatic normoblasts and nucleated RBCs as determined by primary transcript RNA FISH
. | Alleles analyzed, no. . | Percentage expressing alleles . | Standard error . |
|---|---|---|---|
| WT | 152 | 86 | 2.8 |
| ΔHS-62.5, Δ3′HS1 | 150 | 87 | 2.7 |
. | Alleles analyzed, no. . | Percentage expressing alleles . | Standard error . |
|---|---|---|---|
| WT | 152 | 86 | 2.8 |
| ΔHS-62.5, Δ3′HS1 | 150 | 87 | 2.7 |
Fetal liver cells of WT and homozygous ΔHS-62.5, Δ3′HS1 mice were isolated, stained, fractionated, and subjected to primary transcript FISH as described.39 Cells staining with TER-119, but not with antibodies to CD117 or ERY-1, representing the most mature nucleated erythroid cells, were analyzed.