Effect of the CD11b phosphorylation site mutant on leukocyte extravasation in vivo
. | Percentage of injected dose/g tissue . | . | |
|---|---|---|---|
| Organ . | Wt CD11b/Jβ2.7 . | S1126A-CD11b/Jβ2.7 . | |
| Blood | 30 ± 4.7 | 42.2 ± 2.46 | |
| Heart | 17.1 ± 5.5 | 18.38 ± 4.98 | |
| Liver | 12.23 ± 2.16 | 15.35 ± 8.93 | |
| Kidney | 26.46 ± 2.32 | 24.14 ± 10.13 | |
| Lungs | 100.24 ± 37.75 | 6.02 ± 1.93 | |
| Muscle | 4.88 ± 2.98 | 2.04 ± 0.62 | |
| Femur | 10.96 ± 1.62 | 12.59 ± 3.18 | |
| Brain | 0.89 ± 0.37 | 0.099 ± 0.003 | |
| Spleen | 20.9 ± 4.4 | 2.07 ± 0.16 | |
. | Percentage of injected dose/g tissue . | . | |
|---|---|---|---|
| Organ . | Wt CD11b/Jβ2.7 . | S1126A-CD11b/Jβ2.7 . | |
| Blood | 30 ± 4.7 | 42.2 ± 2.46 | |
| Heart | 17.1 ± 5.5 | 18.38 ± 4.98 | |
| Liver | 12.23 ± 2.16 | 15.35 ± 8.93 | |
| Kidney | 26.46 ± 2.32 | 24.14 ± 10.13 | |
| Lungs | 100.24 ± 37.75 | 6.02 ± 1.93 | |
| Muscle | 4.88 ± 2.98 | 2.04 ± 0.62 | |
| Femur | 10.96 ± 1.62 | 12.59 ± 3.18 | |
| Brain | 0.89 ± 0.37 | 0.099 ± 0.003 | |
| Spleen | 20.9 ± 4.4 | 2.07 ± 0.16 | |
125I-surface-labeled wt and S1126A mutant cells were intravenously administered into Balb/c mice. At 1 hour after inoculation, mice were killed, and organs were harvested, weighed, and γ-counted. The result is given as mean ± SD (n = 3).