Colocalization of TfR and LAMP-1 in ARH-77 cells incubated with 11 nM anti–hTfR IgG3-Av
. | Experiment 1 . | . | . | . | Experiment 2 . | . | . | Experiment 3 . | . | . | . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Incubation time, min . | 0 . | 15 . | 30 . | 90 . | 0 . | 30 . | 60 . | 0 . | 15 . | 30 . | 45 . | ||||||||
| Average % colocalization | 10.2 | 20.0 | 16.3 | 18.2 | 13.6 | 20.8 | 18.7 | 11.9 | 21.7 | 19.2 | 39.8 | ||||||||
| SD | 1.5 | 4.0 | 2.6 | 3.4 | 3.8 | 3.0 | 3.9 | 3.5 | 8.1 | 1.4 | 9.8 | ||||||||
| SE | 0.5 | 1.4 | 0.9 | 1.4 | 1.4 | 1.1 | 1.5 | 1.0 | 2.5 | 0.5 | 3.1 | ||||||||
| No. of fields | 11 | 8 | 8 | 6 | 7 | 8 | 7 | 12 | 10 | 9 | 10 | ||||||||
| Average no. of cells/field | 17 | 14 | 16 | 18 | 19 | 21 | 24 | 19 | 11 | 17 | 7 | ||||||||
| P | — | < .001 | < .001 | < .002 | — | < .002 | < .03 | — | < .005 | < .001 | < .001 | ||||||||
. | Experiment 1 . | . | . | . | Experiment 2 . | . | . | Experiment 3 . | . | . | . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Incubation time, min . | 0 . | 15 . | 30 . | 90 . | 0 . | 30 . | 60 . | 0 . | 15 . | 30 . | 45 . | ||||||||
| Average % colocalization | 10.2 | 20.0 | 16.3 | 18.2 | 13.6 | 20.8 | 18.7 | 11.9 | 21.7 | 19.2 | 39.8 | ||||||||
| SD | 1.5 | 4.0 | 2.6 | 3.4 | 3.8 | 3.0 | 3.9 | 3.5 | 8.1 | 1.4 | 9.8 | ||||||||
| SE | 0.5 | 1.4 | 0.9 | 1.4 | 1.4 | 1.1 | 1.5 | 1.0 | 2.5 | 0.5 | 3.1 | ||||||||
| No. of fields | 11 | 8 | 8 | 6 | 7 | 8 | 7 | 12 | 10 | 9 | 10 | ||||||||
| Average no. of cells/field | 17 | 14 | 16 | 18 | 19 | 21 | 24 | 19 | 11 | 17 | 7 | ||||||||
| P | — | < .001 | < .001 | < .002 | — | < .002 | < .03 | — | < .005 | < .001 | < .001 | ||||||||
— indicates not applicable