Engraftment in passive transferred recipients
Group . | Serum, μL*day –1 . | BMT day 0 . | n . | Engraftment, % . |
|---|---|---|---|---|
| A | None | 15 × 106 | 12 | 10 of 12 |
| B | Naive, 500 | 15 × 106 | 8 | 7 of 8 |
| C | Sensitized, 500 | 15 × 106 | 7 | 0 of 7 |
| D | Sensitized, 500 | 40 × 106 | 8 | 0 of 8 |
| E | Sensitized, 200 | 15 × 106 | 4 | 0 of 4 |
| F | Sensitized, 50 | 15 × 106 | 7 | 0 of 7 |
| G | Sensitized, 25 | 15 × 106 | 5 | 1 of 5 |
| H | Sensitized, 10 | 15 × 106 | 6 | 5 of 6 |
Group . | Serum, μL*day –1 . | BMT day 0 . | n . | Engraftment, % . |
|---|---|---|---|---|
| A | None | 15 × 106 | 12 | 10 of 12 |
| B | Naive, 500 | 15 × 106 | 8 | 7 of 8 |
| C | Sensitized, 500 | 15 × 106 | 7 | 0 of 7 |
| D | Sensitized, 500 | 40 × 106 | 8 | 0 of 8 |
| E | Sensitized, 200 | 15 × 106 | 4 | 0 of 4 |
| F | Sensitized, 50 | 15 × 106 | 7 | 0 of 7 |
| G | Sensitized, 25 | 15 × 106 | 5 | 1 of 5 |
| H | Sensitized, 10 | 15 × 106 | 6 | 5 of 6 |
Serum from B10 mice sensitized to B10.BR alloantigens was collected 4 to 6 weeks after sensitization, pooled, decomplemented, and injected into naive B10 recipients. Recipients were conditioned with 700 cGy TBI and underwent transplantation with 15 × 106 or 40 × 106 BMCs 4 to 6 hours after TBI