Effect of forced expression of STAT3-C on CRU maintenance in vitro
Transduction/no. of GFP+ cells injected* . | % positively engrafted mice . | CRU frequency*(95% CI) . |
|---|---|---|
| MIG | 1/1 290 000 (1/5 300 000-1/310 000) | |
| 4.8 × 105 | 20† | |
| 9.6 × 104 | 20† | |
| STAT3-C | 1/410 000 (1/1 340 000-1/130 000) | |
| 3.8 × 105 | 60† | |
| 3.8 × 104 | 0‡ | |
| 7.6 × 103 | 0† |
Transduction/no. of GFP+ cells injected* . | % positively engrafted mice . | CRU frequency*(95% CI) . |
|---|---|---|
| MIG | 1/1 290 000 (1/5 300 000-1/310 000) | |
| 4.8 × 105 | 20† | |
| 9.6 × 104 | 20† | |
| STAT3-C | 1/410 000 (1/1 340 000-1/130 000) | |
| 3.8 × 105 | 60† | |
| 3.8 × 104 | 0‡ | |
| 7.6 × 103 | 0† |
5-FU—pretreated bone marrow cells were transduced with retroviral vectors encoding MIG or Stat3-C. After infection, the cells were cultured for an additional 3 days in medium containing cytokines and 10% FBS. CI indicates confidence interval.
Cell dose representing the day-7 progeny of the number of indicated input cells. Thus, the frequencies shown are directly comparable as they are expressed per starting cell equivalents.
n = 5.
n = 4.