Intracellular reduced folate pools in parental CCRF-CEM cells and their PT523-resistant sublines
Cell line . | CH2THF + THF . | 5-CH3THF . | DHF . | 10-CHO-THF . | Total . |
|---|---|---|---|---|---|
| WT | 24 ± 1 | 9.9 ± 3.1 | 0.3 ± 0.1 | 37 ± 2 | 70 ± 3 |
| PTR0.003 | 19.9 ± 2.7 | 3.6 ± 0.4 | 2.1 ± 1.0 | 18.1 ± 5.5 | 42.4 ± 6.5 |
| PTR0.05 | 13.9 ± 1.3 | 2.4 ± 0.4 | 11.3 ± 1.8 | 7.3 ± 2.4 | 34.9 ± 3.2 |
| PTR0.5 | 1.6 ± 0.3 | 0.4 ± 0.2 | 3.0 ± 0.9 | 0.8 ± 0.3 | 5.8 ± 0.5 |
Cell line . | CH2THF + THF . | 5-CH3THF . | DHF . | 10-CHO-THF . | Total . |
|---|---|---|---|---|---|
| WT | 24 ± 1 | 9.9 ± 3.1 | 0.3 ± 0.1 | 37 ± 2 | 70 ± 3 |
| PTR0.003 | 19.9 ± 2.7 | 3.6 ± 0.4 | 2.1 ± 1.0 | 18.1 ± 5.5 | 42.4 ± 6.5 |
| PTR0.05 | 13.9 ± 1.3 | 2.4 ± 0.4 | 11.3 ± 1.8 | 7.3 ± 2.4 | 34.9 ± 3.2 |
| PTR0.5 | 1.6 ± 0.3 | 0.4 ± 0.2 | 3.0 ± 0.9 | 0.8 ± 0.3 | 5.8 ± 0.5 |
Values represent the mean (pmol/mg protein) ± SEM from 6 determinations performed on 3 separate samples.