Antifolate growth inhibition in parental CCRF-CEM cells and their PT523-resistant sublines
Antifolate . | WT . | PTR0.003 . | PTR0.05 . | PTR0.5 . |
|---|---|---|---|---|
| PT523 | 0.43 ± 0.19 | 3.7 ± 2 (8.7) | 91.7 ± 15.1 (216) | 1471 ± 251 (3461) |
| MTX | 0.86 ± 0.5 | 9.7 ± 5.8 (11.3) | 28.1 ± 16.1 (33) | 512 ± 153 (593) |
| GW1843 | 0.5 ± 0.1 | 2.1 ± 0.4 (4.1) | 26.5 ± 5.3 (53) | 440 ± 167 (881) |
| Pemetrexed (MTA) | 8.1 ± 3.1 | 24.1 ± 0.6 (3) | 208 ± 34.2 (25) | 1063 ± 264 (131) |
| Lometrexol (DDATHF) | 13.5 ± 6.9 | 31.6 ± 8.9 (2.3) | 595 ± 126 (44) | 6945 ± 1362 (514) |
| ZD9331 | 17.6 ± 4.6 | 62 ± 3.9 (3.5) | 874 ± 101 (50) | 4557 ± 984 (259) |
| TMQ | 15.4 ± 6.1 | 3.2 ± 1.4 (0.21) | 2.7 ± 0.5 (0.17) | 1.7 ± 0.4 (0.11) |
Antifolate . | WT . | PTR0.003 . | PTR0.05 . | PTR0.5 . |
|---|---|---|---|---|
| PT523 | 0.43 ± 0.19 | 3.7 ± 2 (8.7) | 91.7 ± 15.1 (216) | 1471 ± 251 (3461) |
| MTX | 0.86 ± 0.5 | 9.7 ± 5.8 (11.3) | 28.1 ± 16.1 (33) | 512 ± 153 (593) |
| GW1843 | 0.5 ± 0.1 | 2.1 ± 0.4 (4.1) | 26.5 ± 5.3 (53) | 440 ± 167 (881) |
| Pemetrexed (MTA) | 8.1 ± 3.1 | 24.1 ± 0.6 (3) | 208 ± 34.2 (25) | 1063 ± 264 (131) |
| Lometrexol (DDATHF) | 13.5 ± 6.9 | 31.6 ± 8.9 (2.3) | 595 ± 126 (44) | 6945 ± 1362 (514) |
| ZD9331 | 17.6 ± 4.6 | 62 ± 3.9 (3.5) | 874 ± 101 (50) | 4557 ± 984 (259) |
| TMQ | 15.4 ± 6.1 | 3.2 ± 1.4 (0.21) | 2.7 ± 0.5 (0.17) | 1.7 ± 0.4 (0.11) |
Values represent IC50, the drug concentration that inhibits cell growth by 50% following a 72-hour antifolate drug exposure. Results depicted are the mean (nM) ± SEM of 3 experiments.
Values in parentheses represent fold of antifolate resistance relative to parental cells.