Table 2. Cell-cycle distribution of... | American Society of Hematology

Table 2.

Cell-cycle distribution of Ba/F3/kit WT and Ba/F3/kit cITD treated with IM and Rap

	Ba/F3/kit WT							Ba/F3/kit cITD1
	Viable cells in phase, %						Viable cells in phase, %
Drugs treated	G₁	S	G₂/M	Total apoptotic cells, %	G₁	S	G₂/M	Total apoptotic cells, %
DMSO, control
24 h	45.8	29.7	22.9	1.6	46.4	23.7	27.1	2.8
48 h	47.2	27.1	23.3	2.4	43.7	23.7	29.2	3.4
50 nM IM
24 h	68.6	7.0	7.2	17.2	46.6	27.2	21.3	4.9
48 h	46.1	2.2	3.8	47.9	60.6	16.7	18	4.7
5 nM Rap
24 h	44.4	25.2	19	11.4	57.4	19.8	17	5.8
48 h	48.9	14.7	11.3	25.1	72.1	9.1	9.5	9.3
50 nM IM and 5 nM Rap
24 h	68.6	7.03	7.2	17.1	68.1	5.9	11.7	14.3
48 h	46.1	2.2	3.78	47.9	46	2.2	3.8	48

	Ba/F3/kit WT							Ba/F3/kit cITD1
	Viable cells in phase, %						Viable cells in phase, %
Drugs treated	G₁	S	G₂/M	Total apoptotic cells, %	G₁	S	G₂/M	Total apoptotic cells, %
DMSO, control
24 h	45.8	29.7	22.9	1.6	46.4	23.7	27.1	2.8
48 h	47.2	27.1	23.3	2.4	43.7	23.7	29.2	3.4
50 nM IM
24 h	68.6	7.0	7.2	17.2	46.6	27.2	21.3	4.9
48 h	46.1	2.2	3.8	47.9	60.6	16.7	18	4.7
5 nM Rap
24 h	44.4	25.2	19	11.4	57.4	19.8	17	5.8
48 h	48.9	14.7	11.3	25.1	72.1	9.1	9.5	9.3
50 nM IM and 5 nM Rap
24 h	68.6	7.03	7.2	17.1	68.1	5.9	11.7	14.3
48 h	46.1	2.2	3.78	47.9	46	2.2	3.8	48

Ba/F3 WT and Ba/F3 kit cITD cells were treated with the indicated doses of IM and Rap, alone and in combination for 24 hours and 48 hours, respectively. Apoptosis was determined via analysis of DNA fragmentation of propidium iodide-stained nuclei

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