Effects of additions of Sema-3A–containing CMs derived from COS-7 clones on cytotoxic activities generated in MLC against K562 cells
. | % 51Cr release at E/T ratio . | . | |
|---|---|---|---|
| Treatment . | 30:1 . | 90:1 . | |
| Medium | 10.0 ± 1.5 | 33.0 ± 2.7 | |
| IL-2, 20 U/mL | 21.0 ± 3.7 | 42.0 ± 4.7 | |
| COS-7/– | 12.0 ± 1.0 | 34.0 ± 5.0 | |
| COS-7/C1 | 7.0 ± 1.0 | 14.0 ± 2.0* | |
| COS-7/C2 | 9.2 ± 1.2 | 20.0 ± 5.3* | |
| COS-7/C3 | 12.0 ± 3.0 | 31.0 ± 6.0 | |
. | % 51Cr release at E/T ratio . | . | |
|---|---|---|---|
| Treatment . | 30:1 . | 90:1 . | |
| Medium | 10.0 ± 1.5 | 33.0 ± 2.7 | |
| IL-2, 20 U/mL | 21.0 ± 3.7 | 42.0 ± 4.7 | |
| COS-7/– | 12.0 ± 1.0 | 34.0 ± 5.0 | |
| COS-7/C1 | 7.0 ± 1.0 | 14.0 ± 2.0* | |
| COS-7/C2 | 9.2 ± 1.2 | 20.0 ± 5.3* | |
| COS-7/C3 | 12.0 ± 3.0 | 31.0 ± 6.0 | |
Mixed lymphocyte cultures (MLCs) were established with 106/mL PBLs and 106/mL allogeneic irradiated (30 Gy [3000 rad]) PBLs supplemented for 6 days with the indicated dose of IL-2 or CMs (1:2 of dilution) derived from COS-7 clones. Lytic activity against 51Cr-labeled erythroid/myeloid cell line K562 (103 cells) was assessed in a 4-hour 51Cr release assay. Data represent the mean ± SE from 2 experiments.
E/T ratio indicates effector-target ratio.
P ≤ .05 versus COS-7/–, ANOVA