Flow cytometric analysis of CACs and EPCs
Marker . | CD14 . | CD45 . | CD31 . | CD34 . | CD105 . | CD144 . | CD146 . | CD163 . | CD64 . |
---|---|---|---|---|---|---|---|---|---|
CAC | + | + | Low | - | - | Low | - | + | + |
EPC | - | - | High | + | + | + | + | - | - |
Marker . | CD14 . | CD45 . | CD31 . | CD34 . | CD105 . | CD144 . | CD146 . | CD163 . | CD64 . |
---|---|---|---|---|---|---|---|---|---|
CAC | + | + | Low | - | - | Low | - | + | + |
EPC | - | - | High | + | + | + | + | - | - |
Cells were harvested on day 7 (CACs) or day 21 (EPCs) of culture, stained with the indicated antibodies, and analyzed by flow cytometry. Unless otherwise indicated, expression was categorized as present (+) or absent (-) based on isotype controls (see Figures 2, 3). A small subpopulation of CACs was CD144+.