Primer sequences for real-time PCR
Gene . | Forward . | Reverse . |
|---|---|---|
| GAPDH | GTCTGGAAAAACCTGCCAAG | ACCTGGTGCTCAGTGTAGCC |
| CD4 | TATGCTGGCTCTGGAAACCT | CTGAGTGGCTCTCATCACCA |
| FoxP3 | GCTGGAGAAGGAGAAGCTGA | CACAGATGAAGCCTTGGTCA |
| CTLA-4 | ATCTGCAAGGTGGAGCTCAT | AATCTGGGTTCCATTGCCTA |
| IDO | ACAGAATGCTGGTGGAGGAC | GGAAGTTCCTGTGAGCTGGT |
| IL-10 | GAGAACCACGACCCAGACAT | CAGCCTGAGGGTCTTCAGAT |
| TGF-β | AACTCACCAGGATGCTCACA | GCACTTCCTCCAGAGGTTTG |
Gene . | Forward . | Reverse . |
|---|---|---|
| GAPDH | GTCTGGAAAAACCTGCCAAG | ACCTGGTGCTCAGTGTAGCC |
| CD4 | TATGCTGGCTCTGGAAACCT | CTGAGTGGCTCTCATCACCA |
| FoxP3 | GCTGGAGAAGGAGAAGCTGA | CACAGATGAAGCCTTGGTCA |
| CTLA-4 | ATCTGCAAGGTGGAGCTCAT | AATCTGGGTTCCATTGCCTA |
| IDO | ACAGAATGCTGGTGGAGGAC | GGAAGTTCCTGTGAGCTGGT |
| IL-10 | GAGAACCACGACCCAGACAT | CAGCCTGAGGGTCTTCAGAT |
| TGF-β | AACTCACCAGGATGCTCACA | GCACTTCCTCCAGAGGTTTG |