Dδ2-Dδ3 rearrangements in individual colonies
. | No. of colonies analyzed . | Dδ2-Dδ3+ colonies . | . | |
|---|---|---|---|---|
. | . | No. . | % . | |
| CFU-GMs from CD34+CD1a- thymocytes | 40 | 4 | 10 | |
| CFU-GMs from CD34+ UCB | 40 | 0 | 0 | |
. | No. of colonies analyzed . | Dδ2-Dδ3+ colonies . | . | |
|---|---|---|---|---|
. | . | No. . | % . | |
| CFU-GMs from CD34+CD1a- thymocytes | 40 | 4 | 10 | |
| CFU-GMs from CD34+ UCB | 40 | 0 | 0 | |
Individual colonies from CFU-GMs were picked, DNA was extracted, and RQ-PCR was done for albumin (to check presence and quality of DNA) and the Dδ2-Dδ3 rearrangements. In all colonies albumin PCR gave appositive signal, the Dδ2-Dδ3 rearrangements only in 4 of 40 colonies from CD34+CD1a- thymocytes.