Peptide characteristics and results of peptide binding assays
Peptide (location) . | Amino acid sequence . | Score,*syfpeithi/bimas . | FI† . | Tetramer . |
|---|---|---|---|---|
| K12 (17-25)‡ | LLNGWRWRL | 25/272 | 0.13 | + |
| ORF8-gB (492-500)§ | LMWYELSKI | 24/244 | 0.83 | + |
| K8.1 (204-213) | GLLLGLVLIL | 32/74 | -0.74 | - |
| K8.1 (209-217)∥ | LVLILYLCV | 20/57 | 0.04 | - |
| LANA (102-110) | ALLPPVTTS | 23/0.6 | 0.55 | - |
| LANA (191-199) | LAPSTLRSL | 22/0.2 | -0.26 | - |
| ORF6 (1050-1058) | VLGDEVLSL | 29/342 | 0.95 | + |
| ORF61 (505-513) | GLADVFAEL | 28/166 | 5.1 | + |
| ORF65 (35-43) | NMSQAEYLV | 19/50 | 0.12 | + |
| ORF65 (129-137) | TLSSGPHSL | 25/21 | 0.05 | - |
| CMV-pp65 | NLVPMVATV | 30/159 | 3.9 | + |
| HIV gag | SLYNTVATL | 31/150 | ND | + |
Peptide (location) . | Amino acid sequence . | Score,*syfpeithi/bimas . | FI† . | Tetramer . |
|---|---|---|---|---|
| K12 (17-25)‡ | LLNGWRWRL | 25/272 | 0.13 | + |
| ORF8-gB (492-500)§ | LMWYELSKI | 24/244 | 0.83 | + |
| K8.1 (204-213) | GLLLGLVLIL | 32/74 | -0.74 | - |
| K8.1 (209-217)∥ | LVLILYLCV | 20/57 | 0.04 | - |
| LANA (102-110) | ALLPPVTTS | 23/0.6 | 0.55 | - |
| LANA (191-199) | LAPSTLRSL | 22/0.2 | -0.26 | - |
| ORF6 (1050-1058) | VLGDEVLSL | 29/342 | 0.95 | + |
| ORF61 (505-513) | GLADVFAEL | 28/166 | 5.1 | + |
| ORF65 (35-43) | NMSQAEYLV | 19/50 | 0.12 | + |
| ORF65 (129-137) | TLSSGPHSL | 25/21 | 0.05 | - |
| CMV-pp65 | NLVPMVATV | 30/159 | 3.9 | + |
| HIV gag | SLYNTVATL | 31/150 | ND | + |
The HLA-A2—restricted CMV-pp65 and HIV-gag peptides were used as positive controls in CMV-infected and HIV-infected individuals, respectively.
ND indicates not determined.
Peptide-binding scores determined using algorithms based on the estimated half-time dissociation of the HLA-peptide complex (www-bimas.dcrt.nih.gov) or prediction of MHC class I ligands.
Fluorescence index (FI) = (MFI with peptide—MFI without peptide)/(MFI without peptide).
Brander et al10 first described this epitope.
Wang et al13 first described this epitope.
Bourboulia et al14 first described this epitope.