TAC1-deficient B cells cannot restore CTL generation in B-cell–deficient mice
B-cell source* . | Response ratio to wild type† . |
|---|---|
| None (controls) | 0.1 |
| Wild-type C57/BL6 B cells | 0.8 |
| β2-/- B cells | 0.9 |
| CD40-/- B cells | 0.9 |
| TAC1-/- B cells | 0.2 |
| IL-2-/- B cells | 0.9 |
| IL-4-/- B cells | 0.9 |
B-cell source* . | Response ratio to wild type† . |
|---|---|
| None (controls) | 0.1 |
| Wild-type C57/BL6 B cells | 0.8 |
| β2-/- B cells | 0.9 |
| CD40-/- B cells | 0.9 |
| TAC1-/- B cells | 0.2 |
| IL-2-/- B cells | 0.9 |
| IL-4-/- B cells | 0.9 |
Purified B cells from different knockout mouse strains were injected intravenously into B-cell—deficient mice 24 hours before CTL priming with the VSV NP 52-59 peptide in IFA.
The wild-type response ratio was calculated by dividing the percent specific lysis at a 10:1 E/T ratio of the VSV NP 52-59—specific CTL response in B-cell—deficient recipient mice to the percent specific lysis observed in untreated wild-type C57/BL6 mice immunized with the same peptide. Data represent the average of 3 independent experiments (SD ≤ 0.01 in all of the groups tested).