Clonogencitiy of SPlow and KLS34- cells
Sorted cell type . | No. wells sorted . | Clonogenicity, % . |
|---|---|---|
| SPlow | 194 | 67 |
| KLS CD34- | 90 | 74 |
| KLS CD34- (+ Hoechst) | 56 | 65 |
Sorted cell type . | No. wells sorted . | Clonogenicity, % . |
|---|---|---|
| SPlow | 194 | 67 |
| KLS CD34- | 90 | 74 |
| KLS CD34- (+ Hoechst) | 56 | 65 |
Single SPlow Sca-1+ cells were sorted in individual wells of a flat bottom 96-well plate containing 100 μL Methocult m3434. Colony number was assessed 12 days after sorting. Two-tailed P values with Fisher exact test: SPlow compared with KLS34- (P < .2); KLS34- compared with Hoechst (P < .7); KLS34- compared with KLS34- with Hoechst (P < .2).