Detection of apoptotic NIH/3T3 cells by fluorescence microscopy
Transfected cells . | p27Kip1wt . | p27Kipl wt c-Abl-PP . | p27Kipl Y74F c-Abl-PP . | p27Kipl Y88/89F c-Abl-PP . | p27Kipl Y74/88/89F c-Abl-PP . |
|---|---|---|---|---|---|
| p27Kip1 positive | |||||
| Counted, no. | 216 | 238 | 168 | 206 | 222 |
| Apoptotic, no. | 4 | 4 | 4 | 6 | 6 |
| Apoptotic, % | 1.85 | 1.68 | 2.38 | 2.91 | 2.70 |
| p27Kip1 negative | |||||
| Counted, no. | 286 | 228 | ND | ND | ND |
| Apoptotic, no. | 6 | 4 | ND | ND | ND |
| Apoptotic, % | 2.09 | 1.75 | ND | ND | ND |
Transfected cells . | p27Kip1wt . | p27Kipl wt c-Abl-PP . | p27Kipl Y74F c-Abl-PP . | p27Kipl Y88/89F c-Abl-PP . | p27Kipl Y74/88/89F c-Abl-PP . |
|---|---|---|---|---|---|
| p27Kip1 positive | |||||
| Counted, no. | 216 | 238 | 168 | 206 | 222 |
| Apoptotic, no. | 4 | 4 | 4 | 6 | 6 |
| Apoptotic, % | 1.85 | 1.68 | 2.38 | 2.91 | 2.70 |
| p27Kip1 negative | |||||
| Counted, no. | 286 | 228 | ND | ND | ND |
| Apoptotic, no. | 6 | 4 | ND | ND | ND |
| Apoptotic, % | 2.09 | 1.75 | ND | ND | ND |
At least 2 independent transfection assays were scored.
p27Kip1 positive indicates transfected cells overexpressing p27Kip1; p27Kip1 negative, transfected cells without p27Kip1 overexpression; ND, not determined.