Frequency of CAFCs in CD45+ fetal liver cell population cultivated on fetal liver stromal cells
. | Pitx2+/+ stroma . | . | Pitx2-/- stroma . | . | ||
|---|---|---|---|---|---|---|
. | CD45+ Pitx2+/+: control . | CD45+ Pitx2-/- . | CD45+ Pitx2+/+ . | CD45+ Pitx2-/- . | ||
| Experiment 1 | 1/48 | 1/53 | 1/110 | 1/174 | ||
| Experiment 2 | 1/17 | 1/16 | 1/142 | 1/96 | ||
| Experiment 3 | 1/28 | 1/39 | 1/211 | 1/151 | ||
| Mean ± SEM | 1/31 ± 15 | 1/36 ± 19 | 1/154* ± 52 | 1/140* ± 40 | ||
. | Pitx2+/+ stroma . | . | Pitx2-/- stroma . | . | ||
|---|---|---|---|---|---|---|
. | CD45+ Pitx2+/+: control . | CD45+ Pitx2-/- . | CD45+ Pitx2+/+ . | CD45+ Pitx2-/- . | ||
| Experiment 1 | 1/48 | 1/53 | 1/110 | 1/174 | ||
| Experiment 2 | 1/17 | 1/16 | 1/142 | 1/96 | ||
| Experiment 3 | 1/28 | 1/39 | 1/211 | 1/151 | ||
| Mean ± SEM | 1/31 ± 15 | 1/36 ± 19 | 1/154* ± 52 | 1/140* ± 40 | ||
The results of 3 quantitative CAFC experiments are shown. For each experiment, CAFC frequency was determined by plotting the proportion of negative wells on a logarithmic scale versus the number of hematopoietic cells seeded per well. Poisson distribution of the CAFCs was verified, and the frequency of CAFCs in the starting cell population was determined as the inverse of the number of seeded cells that corresponds to 37% negative wells (Figure 4 illustrates experiment 3). *Statistically significant differences (P = .01 by Student t test) compared with the control.