Quantification of λ light chain secretion by multiple myeloma cell lines
Cell line . | Secreted λ, μg/1.0 × 106 cells . |
---|---|
8226/S | 29.96 ± 7.39 |
MM.1S | 15.05 ± 4.84 |
Cell line . | Secreted λ, μg/1.0 × 106 cells . |
---|---|
8226/S | 29.96 ± 7.39 |
MM.1S | 15.05 ± 4.84 |
MM cells were cultured at 5.0 × 105 cells/mL for 24 hours, after which the volume of media containing 1.0 × 106 cells was removed from the culture. Cells were pelleted, and the supernatant was removed for ELISA analysis. The data represent the mean ± the SD from 3 independent experiments.