List of primers and probes used for real-time RT-PCR analysis of p16Ink4a and p19Arf mRNA
Gene . | Cdkn2a (p16Ink4a) . | Cdkn2a (p19Arf) . |
|---|---|---|
| Forward primer | CGGTCGTACCC; CGATTCAG | TGAGGCTAGAGAG; GATCTTGAGAAG |
| Reverse primer | GCACCGTAGTT; GAGCAGAAGAG | GTGAACGTTGCCCA; TCATCATC |
| Probe (5′-FAM, 3′-Tamra) | AACGTTGCCCA; TCATCA | ACCTGGTCCAG; GATTC |
| Reference sequence | AF044336 | NM_009877 |
Gene . | Cdkn2a (p16Ink4a) . | Cdkn2a (p19Arf) . |
|---|---|---|
| Forward primer | CGGTCGTACCC; CGATTCAG | TGAGGCTAGAGAG; GATCTTGAGAAG |
| Reverse primer | GCACCGTAGTT; GAGCAGAAGAG | GTGAACGTTGCCCA; TCATCATC |
| Probe (5′-FAM, 3′-Tamra) | AACGTTGCCCA; TCATCA | ACCTGGTCCAG; GATTC |
| Reference sequence | AF044336 | NM_009877 |
All primers and probes were ordered from Applied Biosystems.