Hematologic analysis of whole blood samples from platelet-depleted and control mice following thermal injury
. | . | Absolute cell numbers, × 109/L, mean ± SEM . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| Animal group, by time following injury . | No. mice* . | White blood cells . | Lymphocytes . | Monocytes . | Neutrophils . | |||
| 24 h after injury | ||||||||
| CTL-Ig Sham | 8 | 9.82 ± 0.42 | 8.64 ± 0.42 | 0.44 ± 0.03 | 1.16 ± 0.26 | |||
| CTL-Ig Burn | 7 | 9.34 ± 1.27 | 6.44 ± 1.24 | 0.84 ± 0.14 | 2.54 ± 0.35 | |||
| αPLT-Ig Sham | 8 | 10.29 ± 0.75 | 8.93 ± 0.76 | 0.40 ± 0.04 | 0.68 ± 0.05 | |||
| αPLT-Ig Burn | 9 | 14.50 ± 0.88† | 10.49 ± 0.99† | 1.17 ± 0.15 | 2.02 ± 0.17 | |||
| 36 h after injury | ||||||||
| CTL-Ig Sham | 4 | 6.62 ± 0.48 | 5.72 ± 0.60 | 0.26 ± 0.05 | 0.60 ± 0.05 | |||
| CTL-Ig Burn | 4 | 13.80 ± 1.29 | 10.89 ± 0.49 | 1.26 ± 0.11 | 2.11 ± 0.41 | |||
| αPLT-Ig Sham | 4 | 7.19 ± 0.26 | 6.37 ± 0.21 | 0.22 ± 0.02 | 0.48 ± 0.05 | |||
| αPLT-Ig Burn | 8 | 13.30 ± 0.99 | 8.75 ± 0.62 | 1.79 ± 0.24 | 2.42 ± 0.25 | |||
| 48 h after injury | ||||||||
| CTL-Ig Sham | 8 | 11.30 ± 0.58 | 9.76 ± 0.52 | 0.50 ± 0.03 | 0.94 ± 0.15 | |||
| CTL-Ig Burn | 7 | 5.51 ± 0.34 | 3.70 ± 0.33 | 0.34 ± 0.04 | 0.61 ± 0.06 | |||
| αPLT-Ig Sham | 8 | 9.94 ± 0.87 | 8.82 ± 0.86 | 0.45 ± 0.04 | 0.61 ± 0.06 | |||
| αPLT-Ig Burn | 5 | 6.24 ± 0.51 | 4.51 ± 0.45 | 0.87 ± 0.06 | 0.85 ± 0.04 | |||
. | . | Absolute cell numbers, × 109/L, mean ± SEM . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| Animal group, by time following injury . | No. mice* . | White blood cells . | Lymphocytes . | Monocytes . | Neutrophils . | |||
| 24 h after injury | ||||||||
| CTL-Ig Sham | 8 | 9.82 ± 0.42 | 8.64 ± 0.42 | 0.44 ± 0.03 | 1.16 ± 0.26 | |||
| CTL-Ig Burn | 7 | 9.34 ± 1.27 | 6.44 ± 1.24 | 0.84 ± 0.14 | 2.54 ± 0.35 | |||
| αPLT-Ig Sham | 8 | 10.29 ± 0.75 | 8.93 ± 0.76 | 0.40 ± 0.04 | 0.68 ± 0.05 | |||
| αPLT-Ig Burn | 9 | 14.50 ± 0.88† | 10.49 ± 0.99† | 1.17 ± 0.15 | 2.02 ± 0.17 | |||
| 36 h after injury | ||||||||
| CTL-Ig Sham | 4 | 6.62 ± 0.48 | 5.72 ± 0.60 | 0.26 ± 0.05 | 0.60 ± 0.05 | |||
| CTL-Ig Burn | 4 | 13.80 ± 1.29 | 10.89 ± 0.49 | 1.26 ± 0.11 | 2.11 ± 0.41 | |||
| αPLT-Ig Sham | 4 | 7.19 ± 0.26 | 6.37 ± 0.21 | 0.22 ± 0.02 | 0.48 ± 0.05 | |||
| αPLT-Ig Burn | 8 | 13.30 ± 0.99 | 8.75 ± 0.62 | 1.79 ± 0.24 | 2.42 ± 0.25 | |||
| 48 h after injury | ||||||||
| CTL-Ig Sham | 8 | 11.30 ± 0.58 | 9.76 ± 0.52 | 0.50 ± 0.03 | 0.94 ± 0.15 | |||
| CTL-Ig Burn | 7 | 5.51 ± 0.34 | 3.70 ± 0.33 | 0.34 ± 0.04 | 0.61 ± 0.06 | |||
| αPLT-Ig Sham | 8 | 9.94 ± 0.87 | 8.82 ± 0.86 | 0.45 ± 0.04 | 0.61 ± 0.06 | |||
| αPLT-Ig Burn | 5 | 6.24 ± 0.51 | 4.51 ± 0.45 | 0.87 ± 0.06 | 0.85 ± 0.04 | |||
Anticoagulated whole blood from experimental or control mice was analyzed for absolute cell numbers. Mice per group per time point are listed. Statistical significance of the data (mean ± SEM) was analyzed using the Mann-Whitney test. Differences were considered significant when P < .05.
Animals per group from 3 independent experiments.
P < .025 for CTL-Ig Burn compared with αPLT-Ig Burn.