Competitive hematopoietic repopulation capacity of Lin–Venushigh cells
. | . | No. positive mice/total mice . | . | Chimerism, % . | . | ||
|---|---|---|---|---|---|---|---|
| Cell population . | No. test cells/mouse . | 4 wk . | 12 wk . | 4 wk . | 12 wk . | ||
| Lin-Venushigh | 1 674 | 7/7 | 6/7 | 7.30 ± 1.22 | 17.8 ± 19.6 | ||
| Lin-Venuslow | 17 555 | 0/8 | 0/7 | — | — | ||
| Lin-Venus- | 2 712 | 0/4 | 0/4 | — | — | ||
. | . | No. positive mice/total mice . | . | Chimerism, % . | . | ||
|---|---|---|---|---|---|---|---|
| Cell population . | No. test cells/mouse . | 4 wk . | 12 wk . | 4 wk . | 12 wk . | ||
| Lin-Venushigh | 1 674 | 7/7 | 6/7 | 7.30 ± 1.22 | 17.8 ± 19.6 | ||
| Lin-Venuslow | 17 555 | 0/8 | 0/7 | — | — | ||
| Lin-Venus- | 2 712 | 0/4 | 0/4 | — | — | ||
Lin- cells fractionated by the level of Venus expression (Ly5.1+C57BL/6; test cells) were transplanted into lethally irradiated Ly5.2 C57BL/6 mice with 2 × 105 total BM cells (Ly5.2+ C57BL/6; competitor cells). Peripheral blood cells of recipient mice were analyzed 4 and 12 weeks after transplantation. Percent chimerism (± SD) indicates the percentage of test cells (donor; Ly5.1+) per total Ly5+ (Ly5.1+ + Ly5.2+) cells.
— indicates not engrafted.