Table 4. Western blotting and... | American Society of Hematology

Table 4.

Western blotting and immunocytochemical staining of cell lines for transmembrane adaptor proteins

	LIME			TRIM			NTAL			PAG
	IHC	WB	IHC	WB	IHC	WB	IHC	WB	IHC	WB
B-cell lines
RAJI	Neg	Neg	Neg	Neg	Pos	28 kDa	Pos	90 kDa	Pos	ND
THIEL	Pos	35 kDa	Pos	42 kDa	Pos	28 kDa	Pos^*	90 kDa	Neg	Neg
T-cell lines
CEM	Pos	35 kDa	Pos†	Neg	Neg	Neg	Pos	90 kDa	Pos	ND
HUT-78	Pos	36 kDa	Pos	25 kDa	Neg	ND	Pos	ND	Pos	ND
JURKAT	Neg	ND	Pos	ND	Neg	ND	Neg	ND	Pos‡	ND
Others
KM-H2	Neg	Neg	Neg	Neg	Neg	Neg	Pos	90 kDa	Neg	Neg

	LIME			TRIM			NTAL			PAG
	IHC	WB	IHC	WB	IHC	WB	IHC	WB	IHC	WB
B-cell lines
RAJI	Neg	Neg	Neg	Neg	Pos	28 kDa	Pos	90 kDa	Pos	ND
THIEL	Pos	35 kDa	Pos	42 kDa	Pos	28 kDa	Pos^*	90 kDa	Neg	Neg
T-cell lines
CEM	Pos	35 kDa	Pos†	Neg	Neg	Neg	Pos	90 kDa	Pos	ND
HUT-78	Pos	36 kDa	Pos	25 kDa	Neg	ND	Pos	ND	Pos	ND
JURKAT	Neg	ND	Pos	ND	Neg	ND	Neg	ND	Pos‡	ND
Others
KM-H2	Neg	Neg	Neg	Neg	Neg	Neg	Pos	90 kDa	Neg	Neg

Western blotting was also performed on tonsil-cell lysates, and bands of the same size as those seen in cell lines were observed for LIME, TRIM, NTAL, and PAG. Anti-SIT detected a band with a molecular weight of approximately 35 kDa.

IHC indicates immunohistochemistry; WB, Western blotting; Neg, negative; Pos, positive; ND not done.

In Thiel some cells were PAG⁺

†

In this cell line approximately 50% of the cells were TRIM⁺

‡

In Jurkat some cells were SIT⁺

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