Primers and probes for TaqMan and LUX
Gene . | Designation . | Sequence (5′→3′) . |
|---|---|---|
| ABL | TaqMan forward primer | GATACGAAGGGAGGGTGTACCA |
| ABL | TaqMan reverse primer | CTCGGCCAGGGTGTTGAA |
| ABL | TaqMan probe | TGCTTCTGATGGCAAGCTCTACGTCTCCT |
| ABL | LUX forward primer | caccaaATCAATGGCAGCTTCTTGGtG |
| ABL | LUX reverse primer | CACCCTCCCTTCGTATCTCAGC |
| MPO | LUX forward primer | gtaccgTCGCCTGCATCATCGGtAC |
| MPO | LUX reverse primer | ACCCTCGTTCTCCCACCAAA |
| CD7 | LUX forward primer | cagccTCTCCTTCCTCCTCGGGCtG |
| CD7 | LUX reverse primer | CGCCACGAGCACAGTTTCTT |
| ECP | LUX forward primer | cacagtgCGGGTGCCTTTACTCCACTGtG |
| ECP | LUX reverse primer | ACCTCCTTCCTGGTCTGTCTGC |
| FUT4 | LUX forward primer | gactgcGAGAAAGGTGAGGAGGGCAGtC |
| FUT4 | LUX reverse primer | GCACTTTGCTTCCATTCACAGG |
| HM74 | LUX forward primer | caacacaTGAGATTGGAGGGAATTGTGtTG |
| HM74 | LUX reverse primer | CACGGAAGCCAGGTGACCTAC |
| SMA4 | LUX forward primer | cacggTCTGTATGTGGATGTGATCCGtG |
| SMA4 | LUX reverse primer | AATCAACTCCAGGTGCCCGTA |
Gene . | Designation . | Sequence (5′→3′) . |
|---|---|---|
| ABL | TaqMan forward primer | GATACGAAGGGAGGGTGTACCA |
| ABL | TaqMan reverse primer | CTCGGCCAGGGTGTTGAA |
| ABL | TaqMan probe | TGCTTCTGATGGCAAGCTCTACGTCTCCT |
| ABL | LUX forward primer | caccaaATCAATGGCAGCTTCTTGGtG |
| ABL | LUX reverse primer | CACCCTCCCTTCGTATCTCAGC |
| MPO | LUX forward primer | gtaccgTCGCCTGCATCATCGGtAC |
| MPO | LUX reverse primer | ACCCTCGTTCTCCCACCAAA |
| CD7 | LUX forward primer | cagccTCTCCTTCCTCCTCGGGCtG |
| CD7 | LUX reverse primer | CGCCACGAGCACAGTTTCTT |
| ECP | LUX forward primer | cacagtgCGGGTGCCTTTACTCCACTGtG |
| ECP | LUX reverse primer | ACCTCCTTCCTGGTCTGTCTGC |
| FUT4 | LUX forward primer | gactgcGAGAAAGGTGAGGAGGGCAGtC |
| FUT4 | LUX reverse primer | GCACTTTGCTTCCATTCACAGG |
| HM74 | LUX forward primer | caacacaTGAGATTGGAGGGAATTGTGtTG |
| HM74 | LUX reverse primer | CACGGAAGCCAGGTGACCTAC |
| SMA4 | LUX forward primer | cacggTCTGTATGTGGATGTGATCCGtG |
| SMA4 | LUX reverse primer | AATCAACTCCAGGTGCCCGTA |
The 5 to 7 bases in lower case at the 5′ end of the LUX forward primers are complementary to the 3′ end of the primer to form a hairpin. The single base in lower case at the 3′ end of the primer is the site of fluorophore attachment.