Table 2.

ADAMTS13 antigen and activity levels obtained in the conditioned medium of cells expressing ADAMTS13 variants

Percentage of wt ADAMTS13
Antigen level
Activity level
Construct
Mean
SEM
Mean
SEM
Vector   ND   –   ND   –  
WT   100   –   100   –  
W7   99   5   86   8  
E448   95   8   75   8  
A618   27   4   14   1  
V732   60   5   71   4  
W1336   23   3   12   1  
A-V   7   1   4   0  
W1-A   35   2   9   2  
W1-V   63   12   75   15  
E-A   67   5   28   6  
V-W2  18   2   4   1  
E-A-V   26   2   15   1  
W1-A-V   25   2   12   1  
A-V-W2  4   0   ND   –  
W1-E-A   63   13   32   2  
W1-E-W2  12   1   7   1  
W1-E-A-V   80   8   40   4  
W1-A-V-W2  ND   –   ND   –  
W1-E-A-V-W2  ND   –   ND   –  
X44   ND   –   ND   –  
X44/W1-E-A-V-W2
 		 ND
 		 –
 		 ND
 		 –
 
Percentage of wt ADAMTS13
Antigen level
Activity level
Construct
Mean
SEM
Mean
SEM
Vector   ND   –   ND   –  
WT   100   –   100   –  
W7   99   5   86   8  
E448   95   8   75   8  
A618   27   4   14   1  
V732   60   5   71   4  
W1336   23   3   12   1  
A-V   7   1   4   0  
W1-A   35   2   9   2  
W1-V   63   12   75   15  
E-A   67   5   28   6  
V-W2  18   2   4   1  
E-A-V   26   2   15   1  
W1-A-V   25   2   12   1  
A-V-W2  4   0   ND   –  
W1-E-A   63   13   32   2  
W1-E-W2  12   1   7   1  
W1-E-A-V   80   8   40   4  
W1-A-V-W2  ND   –   ND   –  
W1-E-A-V-W2  ND   –   ND   –  
X44   ND   –   ND   –  
X44/W1-E-A-V-W2
 		 ND
 		 –
 		 ND
 		 –
 

Values were converted to micrograms per 106 cells and units per 106 cells, synchronized according to the calculated β-gal activity, and correlated to wt ADAMTS13 taken as 100%. The mean of ADAMTS13 antigen and activity including SEM (n = 3-10) is depicted. ND indicates not detectable.

– indicates not applicable.

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