Table 1. Erythroid progenitors are... | American Society of Hematology

Table 1.

Erythroid progenitors are reduced in Cul4A^+/- mice but not total bone marrow and erythroid precursors

	No. of cells per femur ± SEM
	Wild-type	*Cul4A*^+/-
Total cells/femur, × 10⁶	32.25 ± 2.23	28.67 ± 2.50
CFU-Es/femur, × 10⁴	1.85 ± 0.41	0.78 ± 0.15*
Erythroid precursors/femur, × 10⁶
Proerythroblasts	0.67 ± 0.11	0.33 ± 0.07
Basophilic erythroblasts	8.11 ± 0.87	7.32 ± 0.47
Late basophilic erythroblasts and polychromatophilic erythroblasts	1.93 ± 0.26	1.57 ± 0.10
Orthochromatophilic erythroblasts	3.59 ± 1.13	2.66 ± 0.29

	No. of cells per femur ± SEM
	Wild-type	*Cul4A*^+/-
Total cells/femur, × 10⁶	32.25 ± 2.23	28.67 ± 2.50
CFU-Es/femur, × 10⁴	1.85 ± 0.41	0.78 ± 0.15*
Erythroid precursors/femur, × 10⁶
Proerythroblasts	0.67 ± 0.11	0.33 ± 0.07
Basophilic erythroblasts	8.11 ± 0.87	7.32 ± 0.47
Late basophilic erythroblasts and polychromatophilic erythroblasts	1.93 ± 0.26	1.57 ± 0.10
Orthochromatophilic erythroblasts	3.59 ± 1.13	2.66 ± 0.29

Cells were isolated from the bone marrow of wild-type and Cul4A^+/- mice. Total bone marrow cells were determined with a Beckman Coulter particle counter. CFU-E colonies were counted 3 days after initiation of the progenitor cell methylcellulose colony assay. Bone marrow cells were incubated with PE-conjugated anti-Ter119 and FITC-conjugated anti-CD71 antibodies. Using flow cytometry, 4 cell populations of erythroid precursors were defined with specific staining characteristics: proerythroblasts as Ter119^med CD71^high; basophilic erythroblasts as Ter119^high CD71^high; late basophilic erythroblasts and polychromatophilic erythroblasts as Ter119^highCD71^med; and orthochromatophilic erythroblasts as Ter119^highCD71^low. The data presented are from one representative experiment with 3 wild-type and 3 Cul4A^+/- mice of 4 independent experiments. The data are the mean ± SEM.

P < .05.

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