Differences between LSK+ and LSK– cells in proteins that are involved in glycolysis and associated pathways and in protection against DNA damage caused by the production of reactive oxygen species
Type, accession number . | Name . | Peptides found . | LSK-/LSK+ protein ratio . | LSK-/LSK+ mRNA ratio . | Gene symbol . |
|---|---|---|---|---|---|
| Glycolytic pathway | |||||
| P45376 | Aldose reductase | 16 | 0.717 | 1.196 | Akr1b1 |
| P06745 | Glucose phosphate isomerase 1 | 29 | 0.726 | 1.017 | Gpi |
| P05064 | Fructose-bisphosphate aldolase A | 30 | 0.705 | 1.069 | Aldoa |
| P17751 | Triose-phosphate isomerase | 32 | 0.448 | 0.687* | Tpi1 |
| P16858 | Glyceraldehyde 3-phosphate dehydrogenase | 73 | 0.739 | 1.250 | Gapd |
| P09411 | Phosphoglycerate kinase 1 | 29 | 0.617 | 0.560* | Pgk1 |
| P17182 | Alpha enolase | 53 | 0.720 | 0.979 | Enoa |
| P52480 | Pyruvate kinase, M2 isozyme | 64 | 0.655 | 0.834 | Kpy2 |
| P16125 | Lactate dehydrogenase 2, B chain | 9 | 0.581 | 0.824 | Ldhb |
| TCA cycle, respiratory chain | |||||
| Q80Y13 | Malate dehydrogenase 1 (TCA cycle) | 42 | 0.707 | 1.006 | Mdh1 |
| Q9EQK1 | NADP+-specific isocitrate dehydrogenase | 24 | 0.692 | 0.676 | Idh2 |
| Q9CQB4 | Ubiquinol-cytochrome c reductase binding protein | 3 | 1.695 | 0.935 | Uqcrb |
| Pentose-phosphate shunt and purine biosynthesis | |||||
| Q9CQ60 | 6-Phosphogluconolactonase | 11 | 1.638 | 2.062* | Pgls |
| P40142 | Transketolase | 48 | 0.722 | 0.895 | Tkt |
| Q80UH0 | IMP cyclohydrolase | 13 | 0.603 | 0.684* | Atic |
| P50247 | Adenosylhomocysteinase | 16 | 0.679 | 0.565* | Ahcy |
| Q64737 | Trifunctional purine biosynthetic protein adenosine-3 | 4 | 0.784 | 0.743* | Gart |
| Protection against oxidative damage | |||||
| P08228 | Cu/Zn superoxide dismutase | 36 | 0.695 | 0.975 | Sod1 |
| P34884 | Macrophage migration inhibitory factor | 3 | 0.377 | 0.974 | Mif |
| P09671 | Mn superoxide dismutase | 3 | 0.655 | 0.900 | Sod2 |
| P10649 | Glutathione S-transferase Mu 1 | 17 | 0.232 | 0.670* | Gstm1 |
| O09131 | Glutathione transferase omega 1 | 7 | 0.608 | 0.604* | Gsto1 |
| O08709 | Peroxiredoxin 6 | 22 | 0.428 | 1.041 | Prdx6 |
| Q9CPU0 | Lactoylglutathione lyase | 3 | 0.607 | 0.771* | Glo1 |
| P28352 | DNA-(apurinic or apyrimidinic site) lyase | 11 | 0.660 | 0.763* | Apex1 |
| Protection against oxidative damage and alkylating agents | |||||
| P47738 | Aldehyde dehydrogenase | 23 | 0.460 | 0.770* | Aldh2 |
Type, accession number . | Name . | Peptides found . | LSK-/LSK+ protein ratio . | LSK-/LSK+ mRNA ratio . | Gene symbol . |
|---|---|---|---|---|---|
| Glycolytic pathway | |||||
| P45376 | Aldose reductase | 16 | 0.717 | 1.196 | Akr1b1 |
| P06745 | Glucose phosphate isomerase 1 | 29 | 0.726 | 1.017 | Gpi |
| P05064 | Fructose-bisphosphate aldolase A | 30 | 0.705 | 1.069 | Aldoa |
| P17751 | Triose-phosphate isomerase | 32 | 0.448 | 0.687* | Tpi1 |
| P16858 | Glyceraldehyde 3-phosphate dehydrogenase | 73 | 0.739 | 1.250 | Gapd |
| P09411 | Phosphoglycerate kinase 1 | 29 | 0.617 | 0.560* | Pgk1 |
| P17182 | Alpha enolase | 53 | 0.720 | 0.979 | Enoa |
| P52480 | Pyruvate kinase, M2 isozyme | 64 | 0.655 | 0.834 | Kpy2 |
| P16125 | Lactate dehydrogenase 2, B chain | 9 | 0.581 | 0.824 | Ldhb |
| TCA cycle, respiratory chain | |||||
| Q80Y13 | Malate dehydrogenase 1 (TCA cycle) | 42 | 0.707 | 1.006 | Mdh1 |
| Q9EQK1 | NADP+-specific isocitrate dehydrogenase | 24 | 0.692 | 0.676 | Idh2 |
| Q9CQB4 | Ubiquinol-cytochrome c reductase binding protein | 3 | 1.695 | 0.935 | Uqcrb |
| Pentose-phosphate shunt and purine biosynthesis | |||||
| Q9CQ60 | 6-Phosphogluconolactonase | 11 | 1.638 | 2.062* | Pgls |
| P40142 | Transketolase | 48 | 0.722 | 0.895 | Tkt |
| Q80UH0 | IMP cyclohydrolase | 13 | 0.603 | 0.684* | Atic |
| P50247 | Adenosylhomocysteinase | 16 | 0.679 | 0.565* | Ahcy |
| Q64737 | Trifunctional purine biosynthetic protein adenosine-3 | 4 | 0.784 | 0.743* | Gart |
| Protection against oxidative damage | |||||
| P08228 | Cu/Zn superoxide dismutase | 36 | 0.695 | 0.975 | Sod1 |
| P34884 | Macrophage migration inhibitory factor | 3 | 0.377 | 0.974 | Mif |
| P09671 | Mn superoxide dismutase | 3 | 0.655 | 0.900 | Sod2 |
| P10649 | Glutathione S-transferase Mu 1 | 17 | 0.232 | 0.670* | Gstm1 |
| O09131 | Glutathione transferase omega 1 | 7 | 0.608 | 0.604* | Gsto1 |
| O08709 | Peroxiredoxin 6 | 22 | 0.428 | 1.041 | Prdx6 |
| Q9CPU0 | Lactoylglutathione lyase | 3 | 0.607 | 0.771* | Glo1 |
| P28352 | DNA-(apurinic or apyrimidinic site) lyase | 11 | 0.660 | 0.763* | Apex1 |
| Protection against oxidative damage and alkylating agents | |||||
| P47738 | Aldehyde dehydrogenase | 23 | 0.460 | 0.770* | Aldh2 |
Protein accession numbers refer to SWISS-PROT or TrEMBL entries. Confidence score (a percentage measure of the confidence of the protein identification13 ) for all proteins in the table was 99%. Peptides found denote the sum of all different peptides found and relatively quantified from the proteins shown in 3 replicate experiments. All proteins are significantly different (P < .05) between LSK+ cells and LSK- cells.
Significantly different mRNA ratios (P < .05).