Effect of IL-18 administration on donor T-cell responses in primary and secondary MLR
| . | cpm . | IFN-γ, pg/mL . | IL-4, pg/mL . |
|---|---|---|---|
| Primary MLR | |||
| Syngeneic | 247 ± 39 | 38 ± 21 | UD |
| Allogeneic + control | 37 358 ± 1596 | 4473 ± 296 | UD |
| Allogeneic + IL-18 | 32 649 ± 4771 | 3362 ± 958 | UD |
| Seconday MLR | |||
| Syngeneic | 979 ± 254 | 675 ± 342 | 13 ± 6 |
| Allogeneic + control | 76 988 ± 9752 | 16 786 ± 1041 | 23 ± 7 |
| Allogeneic + IL-18 | 48 676 ± 6453* | 9863 ± 474* | 94 ± 12* |
| . | cpm . | IFN-γ, pg/mL . | IL-4, pg/mL . |
|---|---|---|---|
| Primary MLR | |||
| Syngeneic | 247 ± 39 | 38 ± 21 | UD |
| Allogeneic + control | 37 358 ± 1596 | 4473 ± 296 | UD |
| Allogeneic + IL-18 | 32 649 ± 4771 | 3362 ± 958 | UD |
| Seconday MLR | |||
| Syngeneic | 979 ± 254 | 675 ± 342 | 13 ± 6 |
| Allogeneic + control | 76 988 ± 9752 | 16 786 ± 1041 | 23 ± 7 |
| Allogeneic + IL-18 | 48 676 ± 6453* | 9863 ± 474* | 94 ± 12* |
BALB/c mice were injected with IL-18 or diluent (control) for 10 days, as in “Materials and methods.” Splenic T (CD3+) cells were harvested on day 11 and cultured in primary MLR (n = 3/group) with irradiated B6 splenocytes for 96 hours. Culture supernatants were collected for cytokine measurement and proliferation was determined by pulsing with 3H-thymidine (1 μCi/well [0.037 MBq/well]) for an additional 16 hours. Secondary MLR responder T cells from bulk primary MLRs were then removed and restimulated with irradiated B6 peritoneal cells. After 48 hours of culture, supernatants were collected for cytokine measurement, and proliferation was determined by pulsing with3H-thymidine (1 μCi/well [0.037 MBq/well]) for an additional 20 hours. *P < .05.