Transduction with IL-17 into tumor cells markedly augments production of proangiogenic factors of tumor
| Angiogenic factors . | MCA205 . | MCA205Neo . | MCA205IL-174-150 . | MCA205IL-17 + mAb . |
|---|---|---|---|---|
| VEGF | 4.12 ± 0.31 | 4.17 ± 0.25 | 6.87 ± 0.57 | 4.25 ± 0.37 |
| KC | 1.25 ± 0.12 | 1.31 ± 0.17 | 25.27 ± 2.15 | 1.41 ± 0.17 |
| PGE2 | 15.5 ± 1.1 | 14.8 ± 1.3 | 47.5 ± 3.2 | 16.1 ± 1.5 |
| Angiogenic factors . | MCA205 . | MCA205Neo . | MCA205IL-174-150 . | MCA205IL-17 + mAb . |
|---|---|---|---|---|
| VEGF | 4.12 ± 0.31 | 4.17 ± 0.25 | 6.87 ± 0.57 | 4.25 ± 0.37 |
| KC | 1.25 ± 0.12 | 1.31 ± 0.17 | 25.27 ± 2.15 | 1.41 ± 0.17 |
| PGE2 | 15.5 ± 1.1 | 14.8 ± 1.3 | 47.5 ± 3.2 | 16.1 ± 1.5 |
Cells (1 × 105/mL) were cultured for 48 hours in CM with or without 20 μg/mL neutralizing antimouse IL-17 mAb. Cell-free supernatants were collected and assayed for the concentrations of VEGF, KC, and PGE2 (ng/mL) by ELISA. MCA205 cells transduced with IL-17 produced more VEGF, KC, and PGE2 in response to IL-17 produced by themselves. Data were expressed as mean ± SD (n = 3 per data point).
P < .05.