IL-17 markedly augments production of proangiogenic factors of fibroblast
| Angiogenic factors . | Fibroblast . | Fibroblast + IL-173-150 . | Fibroblast + IL-17 + mAb . |
|---|---|---|---|
| VEGF | 2.82 ± 0.18 | 4.69 ± 0.33 | 2.77 ± 0.23 |
| KC | 1.3 ± 0.05 | 26.5 ± 2.15 | 1.41 ± 0.12 |
| MIP-2 | 36.0 ± 1.3 | 52.0 ± 2.2 | 37.7 ± 1.5 |
| PGE1 | 0.8 ± 0.35 | 4.8 ± 0.65 | 0.87 ± 0.33 |
| PGE2 | 39.5 ± 2.45 | 81.1 ± 7.35 | 38.7 ± 2.86 |
| NO | 8.1 ± 0.95 | 13.3 ± 1.0 | 8.21 ± 1.1 |
| Angiogenic factors . | Fibroblast . | Fibroblast + IL-173-150 . | Fibroblast + IL-17 + mAb . |
|---|---|---|---|
| VEGF | 2.82 ± 0.18 | 4.69 ± 0.33 | 2.77 ± 0.23 |
| KC | 1.3 ± 0.05 | 26.5 ± 2.15 | 1.41 ± 0.12 |
| MIP-2 | 36.0 ± 1.3 | 52.0 ± 2.2 | 37.7 ± 1.5 |
| PGE1 | 0.8 ± 0.35 | 4.8 ± 0.65 | 0.87 ± 0.33 |
| PGE2 | 39.5 ± 2.45 | 81.1 ± 7.35 | 38.7 ± 2.86 |
| NO | 8.1 ± 0.95 | 13.3 ± 1.0 | 8.21 ± 1.1 |
Cells (1 × 105/mL) were cultured for 48 hours with or without 50 ng/mL IL-17 or 50 ng/mL IL-17 plus 20 μg/mL neutralizing antimouse IL-17 mAb. Cell-free supernatants were collected and assayed for the concentrations of VEGF, KC, MIP-2, PGE1, PGE2, and NO (ng/mL for VEGF, KC, PGE1, and PGE2; pg/mL for MIP-2; μM for NO). Data were expressed as mean ± SD (n = 3 per data point).
P < .05.