IL-6 production is markedly increased in IL-17–transfected tumor cell lines
| Cell lines . | IL-6 (pg/mL) . |
|---|---|
| MCA205WT | < 7.8 |
| MCA205Neo | < 7.8 |
| MCA205IL-17 | 238 |
| MCA205IL-17 + mAb | 11 |
| MC38WT | < 7.8 |
| MC38Neo | < 7.8 |
| MC38IL-17 | 146 |
| MC38IL-17 + mAb | < 7.8 |
| Cell lines . | IL-6 (pg/mL) . |
|---|---|
| MCA205WT | < 7.8 |
| MCA205Neo | < 7.8 |
| MCA205IL-17 | 238 |
| MCA205IL-17 + mAb | 11 |
| MC38WT | < 7.8 |
| MC38Neo | < 7.8 |
| MC38IL-17 | 146 |
| MC38IL-17 + mAb | < 7.8 |
Cells (1 × 105/mL) were cultured in CM with or without 20 μg/mL antimouse IL-17 monoclonal antibody. Cell-free supernatants were collected after 48 hours and assayed for the production of IL-6 by ELISA. Tumor cells transduced withIL-17 gene produced more IL-6 in response to IL-17 produced by themselves.