ICAM-1 and PECAM-1 expression in cytokine and chemokine-treated REN and RHP cells
| Cell line (protein) . | No Rx . | TNFα, 24 h . | IL-8, 4 h . | IL-1β, 24 h . |
|---|---|---|---|---|
| REN (ICAM-1) | 1.0 (49.2) | 2.73 (134.7) | 1.0 (49.5) | 2.22 (109.3) |
| RHP (ICAM-1) | 1.0 (68.1) | 2.12 (144.4) | 0.95 (64.9) | 2.45 (168.8) |
| (PECAM-1) | 1.0 (42.3) | 0.91 (38.4) | — | 1.01 (42.7) |
| Cell line (protein) . | No Rx . | TNFα, 24 h . | IL-8, 4 h . | IL-1β, 24 h . |
|---|---|---|---|---|
| REN (ICAM-1) | 1.0 (49.2) | 2.73 (134.7) | 1.0 (49.5) | 2.22 (109.3) |
| RHP (ICAM-1) | 1.0 (68.1) | 2.12 (144.4) | 0.95 (64.9) | 2.45 (168.8) |
| (PECAM-1) | 1.0 (42.3) | 0.91 (38.4) | — | 1.01 (42.7) |
ICAM-1 and PECAM-1 surface expression in cell monolayers treated for 24 hours with IL-1β or TNFα (cytokines) or for 4 hours with IL-8 (chemokine); conditions were identical to transmigration experiments. Representative experiment evaluating protein expression in cells analyzed by FACS. Mean fluorescence intensity values are normalized to baseline ICAM-1 or PECAM-1 expression levels for each cell line (mean fluorescence intensity). Similar results were obtained in at least 3 replicate experiments for each condition. No Rx indicates no treatment; —, not assessed.