In vitro evaluation of tolerance through mixed lymphocyte reaction assays
| Group . | Stimulation index . | D:3rd . | ||
|---|---|---|---|---|
| Antihost . | Antidonor . | Anti–third party . | ||
| Normal B6 | 1.5 ± 0.3 | 5.8 ± 7.0 | 2.4 ± 0.9 | 2.1 ± 1.3 |
| Normal Balb/c | 15.5 ± 9.8 | 1.9 ± 0.9 | 4.3 ± 2.1 | 0.5 ± 0.2 |
| BMT + cobl* | 1.5 ± 0.4 | 1.4 ± 0.6 | 2.3 ± 1.3 | 0.7 ± 0.4 |
| + CyA | 1.5 ± 0.5 | 7.3 ± 6.0 | 2.4 ± 0.4 | 3.1 ± 2.6 |
| + FK | 1.4 ± 0.2 | 2.6 ± 2.3 | 1.9 ± 0.9 | 1.2 ± 0.5 |
| + Rapa | 2.5 ± 0.9 | 1.7 ± 0.6 | 3.6 ± 0.6 | 0.5 ± 0.1 |
| + MP | 1.6 ± 0.6 | 1.1 ± 0.2 | 2.3 ± 1.4 | 0.6 ± 0.3 |
| + FTY720 | 1.6 ± 0.4 | 1.3 ± 0.3 | 2.4 ± 1.1 | 0.6 ± 0.2 |
| + MMF | 1.6 ± 0.9 | 1.6 ± 1.2 | 2.6 ± 1.8 | 0.8 ± 0.7 |
| Group . | Stimulation index . | D:3rd . | ||
|---|---|---|---|---|
| Antihost . | Antidonor . | Anti–third party . | ||
| Normal B6 | 1.5 ± 0.3 | 5.8 ± 7.0 | 2.4 ± 0.9 | 2.1 ± 1.3 |
| Normal Balb/c | 15.5 ± 9.8 | 1.9 ± 0.9 | 4.3 ± 2.1 | 0.5 ± 0.2 |
| BMT + cobl* | 1.5 ± 0.4 | 1.4 ± 0.6 | 2.3 ± 1.3 | 0.7 ± 0.4 |
| + CyA | 1.5 ± 0.5 | 7.3 ± 6.0 | 2.4 ± 0.4 | 3.1 ± 2.6 |
| + FK | 1.4 ± 0.2 | 2.6 ± 2.3 | 1.9 ± 0.9 | 1.2 ± 0.5 |
| + Rapa | 2.5 ± 0.9 | 1.7 ± 0.6 | 3.6 ± 0.6 | 0.5 ± 0.1 |
| + MP | 1.6 ± 0.6 | 1.1 ± 0.2 | 2.3 ± 1.4 | 0.6 ± 0.3 |
| + FTY720 | 1.6 ± 0.4 | 1.3 ± 0.3 | 2.4 ± 1.1 | 0.6 ± 0.2 |
| + MMF | 1.6 ± 0.9 | 1.6 ± 1.2 | 2.6 ± 1.8 | 0.8 ± 0.7 |
CyA- and FK-treated mice demonstrate increased donor reactivity compared with mice receiving the standard protocol or additional rapa, MP, FTY720, or MMF. Combined results from 2 experiments are shown as mean stimulation index (SI) ± SD. Three to 6 mice per group were analyzed. SIs were calculated by dividing the mean cpm from responses against host (B6), donor (Balb/c), or third-party (C3H) stimulator cells by mean background cpm (ie, cpm with no stimulator population). D:3rd denotes the ratio of antidonor to anti–third-party SI.
Standard-protocol group.