In vitro effect of 4F on CD34+ cell cycle distribution
| % CD34+ cells . | Day 0 . | Day 1 . | Day 3 . | ||
|---|---|---|---|---|---|
| — . | Unirradiated . | 2.5 Gy . | Unirradiated . | 2.5 Gy . | |
| G0 | 67.8 ± 5.8 | 40.2 ± 12.6 | 78.4 ± 7.9 | 4.6 ± 4.3 | 7.8 ± 3.7 |
| G1 | 29.9 ± 4.8 | 39.5 ± 6.0 | 14.3 ± 6.8 | 55.3 ± 14.7 | 53.1 ± 9.5 |
| S/G2/M | 2.4 ± 1.2 | 19.8 ± 7.6 | 6.5 ± 3.2 | 40.0 ± 12.2 | 37.3 ± 7.0 |
| % CD34+ cells . | Day 0 . | Day 1 . | Day 3 . | ||
|---|---|---|---|---|---|
| — . | Unirradiated . | 2.5 Gy . | Unirradiated . | 2.5 Gy . | |
| G0 | 67.8 ± 5.8 | 40.2 ± 12.6 | 78.4 ± 7.9 | 4.6 ± 4.3 | 7.8 ± 3.7 |
| G1 | 29.9 ± 4.8 | 39.5 ± 6.0 | 14.3 ± 6.8 | 55.3 ± 14.7 | 53.1 ± 9.5 |
| S/G2/M | 2.4 ± 1.2 | 19.8 ± 7.6 | 6.5 ± 3.2 | 40.0 ± 12.2 | 37.3 ± 7.0 |
Unirradiated (unir) and in vitro–irradiated baboon-mobilized PB CD34+ cells were incubated for 7 days in serum-free medium in the presence of 4F, and cell cycle analysis was performed by flow cytometry after 1 or 3 days of culture. Results from 4 different baboons are expressed as mean ± SEM. All intergroup comparisons, made using the Mann-Whitney U test, are significant (P < .05) except the following: day 0 versus day 1, 2.5 Gy; day 3 unir versus day 3, 2.5 Gy regarding both G0 and S/G2/M; day 3 unir versus day 3, 2.5 Gy regarding G1.