Flow cytometric analysis of BM myeloid cells
| . | CIN patients . | Healthy controls . | P* . |
|---|---|---|---|
| BMMC fraction | |||
| % CD34+cells | 1.01 ± 0.50 | 2.04 ± 0.50 | < .0001 |
| Median (range) | 0.85 (0.20-2.60) | 1.84 (0.70-4.80) | |
| No. | 32 | 46 | |
| % CD34+/CD33+cells | 0.11 ± 0.05 | 0.36 ± 0.23 | < .0001 |
| Median (range) | 0.10 (0.05-0.20) | 0.40 (0.10-0.90) | |
| No. | 30 | 13 | |
| % CD34+/CD33−cells | 0.84 ± 0.39 | 1.43 ± 0.84 | .0039 |
| Median (range) | 0.70 (0.35-1.70) | 0.90 (0.60-2.90) | |
| No. | 30 | 13 | |
| CD34+cell fraction | |||
| % Fas+cells | 12.26 ± 9.90 | 6.36 ± 3.24 | .0043 |
| Median (range) | 9.20 (3.10-48.70) | 6.60 (0.80-11.90) | |
| No. | 31 | 28 | |
| % 7-AAD+cells | 11.28 ± 9.87 | 6.60 ± 4.96 | .0189 |
| Median (range) | 7.60 (3.00-49.70) | 5.04 (1.30-20.89) | |
| No. | 31 | 29 | |
| CD34+/CD33−-purified cells | |||
| % Fas+cells | 4.10 ± 4.77 | 1.11 ± 0.66 | .0612 |
| Median (range) | 1.90 (0.30-15.10) | 0.95 (0.30-2.10) | |
| No. | 18 | 10 | |
| % 7AAD+cells | 7.59 ± 12.60 | 2.92 ± 4.20 | .2686 |
| Median (range) | 3.30 (0.30-31.60) | 1.40 (0.20-13.70) | |
| No. | 18 | 10 | |
| CD34+/CD33+-purified cells | |||
| % Fas+cells | 21.38 ± 15.68 | 6.09 ± 4.38 | .0059 |
| Median (range) | 24.85 (3.10-56.80) | 4.45 (1.00-13.00) | |
| No. | 18 | 10 | |
| % 7-AAD+cells | 18.44 ± 15.45 | 3.23 ± 3.97 | .0054 |
| Median (range) | 15.40 (0.80-51.90) | 1.60 (0.40-10.40) | |
| No. | 18 | 10 | |
| CD34−/CD33+-purified cells | |||
| % Fas+cells | 69.62 ± 17.02 | 81.56 ± 11.65 | .0596 |
| Median (range) | 72.35 (20.80-93.20) | 79.00 (66.00-97.60) | |
| No. | 18 | 10 | |
| % 7-AAD+cells | 8.02 ± 5.30 | 5.87 ± 2.84 | .2465 |
| Median (range) | 5.30 (2.30-22.40) | 6.85 (0.40-10.30) | |
| No. | 18 | 10 | |
| CD33−/CD15+-purified cells | |||
| % Fas+cells | 28.57 ± 21.03 | 40.00 ± 21.75 | .1851 |
| Median (range) | 26.00 (3.00-69.30) | 41.65 (11.50-68.80) | |
| No. | 18 | 10 | |
| % 7-AAD+cells | 6.31 ± 3.14 | 3.90 ± 2.93 | .0570 |
| Median (range) | 5.40 (2.90-14.00) | 3.80 (0.30-9.50) | |
| No. | 18 | 10 |
| . | CIN patients . | Healthy controls . | P* . |
|---|---|---|---|
| BMMC fraction | |||
| % CD34+cells | 1.01 ± 0.50 | 2.04 ± 0.50 | < .0001 |
| Median (range) | 0.85 (0.20-2.60) | 1.84 (0.70-4.80) | |
| No. | 32 | 46 | |
| % CD34+/CD33+cells | 0.11 ± 0.05 | 0.36 ± 0.23 | < .0001 |
| Median (range) | 0.10 (0.05-0.20) | 0.40 (0.10-0.90) | |
| No. | 30 | 13 | |
| % CD34+/CD33−cells | 0.84 ± 0.39 | 1.43 ± 0.84 | .0039 |
| Median (range) | 0.70 (0.35-1.70) | 0.90 (0.60-2.90) | |
| No. | 30 | 13 | |
| CD34+cell fraction | |||
| % Fas+cells | 12.26 ± 9.90 | 6.36 ± 3.24 | .0043 |
| Median (range) | 9.20 (3.10-48.70) | 6.60 (0.80-11.90) | |
| No. | 31 | 28 | |
| % 7-AAD+cells | 11.28 ± 9.87 | 6.60 ± 4.96 | .0189 |
| Median (range) | 7.60 (3.00-49.70) | 5.04 (1.30-20.89) | |
| No. | 31 | 29 | |
| CD34+/CD33−-purified cells | |||
| % Fas+cells | 4.10 ± 4.77 | 1.11 ± 0.66 | .0612 |
| Median (range) | 1.90 (0.30-15.10) | 0.95 (0.30-2.10) | |
| No. | 18 | 10 | |
| % 7AAD+cells | 7.59 ± 12.60 | 2.92 ± 4.20 | .2686 |
| Median (range) | 3.30 (0.30-31.60) | 1.40 (0.20-13.70) | |
| No. | 18 | 10 | |
| CD34+/CD33+-purified cells | |||
| % Fas+cells | 21.38 ± 15.68 | 6.09 ± 4.38 | .0059 |
| Median (range) | 24.85 (3.10-56.80) | 4.45 (1.00-13.00) | |
| No. | 18 | 10 | |
| % 7-AAD+cells | 18.44 ± 15.45 | 3.23 ± 3.97 | .0054 |
| Median (range) | 15.40 (0.80-51.90) | 1.60 (0.40-10.40) | |
| No. | 18 | 10 | |
| CD34−/CD33+-purified cells | |||
| % Fas+cells | 69.62 ± 17.02 | 81.56 ± 11.65 | .0596 |
| Median (range) | 72.35 (20.80-93.20) | 79.00 (66.00-97.60) | |
| No. | 18 | 10 | |
| % 7-AAD+cells | 8.02 ± 5.30 | 5.87 ± 2.84 | .2465 |
| Median (range) | 5.30 (2.30-22.40) | 6.85 (0.40-10.30) | |
| No. | 18 | 10 | |
| CD33−/CD15+-purified cells | |||
| % Fas+cells | 28.57 ± 21.03 | 40.00 ± 21.75 | .1851 |
| Median (range) | 26.00 (3.00-69.30) | 41.65 (11.50-68.80) | |
| No. | 18 | 10 | |
| % 7-AAD+cells | 6.31 ± 3.14 | 3.90 ± 2.93 | .0570 |
| Median (range) | 5.40 (2.90-14.00) | 3.80 (0.30-9.50) | |
| No. | 18 | 10 |
All values are expressed as mean ± 1 SD.
Comparison of values between patients and healthy controls was performed with the Student t test. P ≤ .05 was considered statistically significant.