Primers and PCR conditions for determination of genotype and TCA of 5 X-chromosome markers
| Gene . | Primer . | Primer sequence (5′-3′) . | MgCl2, mM . | DMSO, % . | PCR product, bp . |
|---|---|---|---|---|---|
| BTK | D-For | GTGAGACTGCTGAACACATTG | 3 | 5 | 156 |
| D-Rev | GCTAAATGGGCAAGTAGATTC | ||||
| R-For | GTGAGACTGCTGAACACATTG | 1 | 0 | 172 | |
| R-Rev | GCTCAGGATTCTTCATCCATG | ||||
| FHL1 | D-For | AGCCCCCTC AGATGTTCC | 3 | 10 | 149 |
| D-Rev | CTACGTTACAA GGGATTCAACC | ||||
| R-For | AGCCCCCTC AGATGTTCC | 1 | 0 | 149 | |
| R-Rev | CTACGTTACAA GGGATTCAACC | ||||
| MPP1 | D-For | TCTAAAATA ACCTCAGAA AGAG | 3 | 0 | 130 |
| D-Rev | GGATCATGCCACCA TGAAGA | ||||
| R-For | GACAGGAGGTGCGGAAAGTG | 1 | 0 | 129 | |
| R-Rev | GGATCATGCCACCATGAAGA | ||||
| G6PD | D-For | CAGTGGCATCAGCAAGACAC | 1.5 | 5 | 151 |
| D-Rev | CATAGCCCA CAGGTATGCAGG | ||||
| R-For | CTGGACCTGACCTACGGCA | 1 | 0 | 147 | |
| R-Rev | CAGTGGGGTGAAAATACGC | ||||
| IDS | R-For | GGAGAATGGCTATGTGACC | 1 | 0 | 159 |
| R-Rev | TCTCCATCTGGCCCTCGAC |
| Gene . | Primer . | Primer sequence (5′-3′) . | MgCl2, mM . | DMSO, % . | PCR product, bp . |
|---|---|---|---|---|---|
| BTK | D-For | GTGAGACTGCTGAACACATTG | 3 | 5 | 156 |
| D-Rev | GCTAAATGGGCAAGTAGATTC | ||||
| R-For | GTGAGACTGCTGAACACATTG | 1 | 0 | 172 | |
| R-Rev | GCTCAGGATTCTTCATCCATG | ||||
| FHL1 | D-For | AGCCCCCTC AGATGTTCC | 3 | 10 | 149 |
| D-Rev | CTACGTTACAA GGGATTCAACC | ||||
| R-For | AGCCCCCTC AGATGTTCC | 1 | 0 | 149 | |
| R-Rev | CTACGTTACAA GGGATTCAACC | ||||
| MPP1 | D-For | TCTAAAATA ACCTCAGAA AGAG | 3 | 0 | 130 |
| D-Rev | GGATCATGCCACCA TGAAGA | ||||
| R-For | GACAGGAGGTGCGGAAAGTG | 1 | 0 | 129 | |
| R-Rev | GGATCATGCCACCATGAAGA | ||||
| G6PD | D-For | CAGTGGCATCAGCAAGACAC | 1.5 | 5 | 151 |
| D-Rev | CATAGCCCA CAGGTATGCAGG | ||||
| R-For | CTGGACCTGACCTACGGCA | 1 | 0 | 147 | |
| R-Rev | CAGTGGGGTGAAAATACGC | ||||
| IDS | R-For | GGAGAATGGCTATGTGACC | 1 | 0 | 159 |
| R-Rev | TCTCCATCTGGCCCTCGAC |
PCR primers for genotyping (D-For, D-Rev); RT-PCR primers for TCA (R-For, R-Rev).