Addition of antimurine Tpo-neutralizing antibody to cultures has no effect on baseline CFU counts
| . | EryP . | BFU-E . | CFU-Mix . | CFU-Meg . |
|---|---|---|---|---|
| GF only | 474.5 ± 40.0 | 10.3 ± 0.5 | 19.8 ± 0.8 | 0.5 ± 0.3 |
| GF + hTpo | 235.0 ± 22.3 | 5.3 ± 0.5 | 33.3 ± 2.5 | 6.0 ± 0.4 |
| GF + anti-mTpo | 474.5 ± 35.6 | 12.0 ± 0.9 | 20.8 ± 1.0 | 0.8 ± 0.3 |
| GF + control | 455.5 ± 30.6 | 10.0 ± 0.9 | 19.0 ± 0.9 | 1.0 ± 0.0 |
| . | EryP . | BFU-E . | CFU-Mix . | CFU-Meg . |
|---|---|---|---|---|
| GF only | 474.5 ± 40.0 | 10.3 ± 0.5 | 19.8 ± 0.8 | 0.5 ± 0.3 |
| GF + hTpo | 235.0 ± 22.3 | 5.3 ± 0.5 | 33.3 ± 2.5 | 6.0 ± 0.4 |
| GF + anti-mTpo | 474.5 ± 35.6 | 12.0 ± 0.9 | 20.8 ± 1.0 | 0.8 ± 0.3 |
| GF + control | 455.5 ± 30.6 | 10.0 ± 0.9 | 19.0 ± 0.9 | 1.0 ± 0.0 |
E8.5 yolk sac cells were used for EryP assay and 10 000 cells/mL of E9.5 yolk sac for other progenitor assays. Antimurine-neutralizing antibody (anti-mTpo) or control antibody concentration was 0.3 μg/mL. GF indicates growth factor.