Molecular weights and sizes of sheared VWF
| Shear applied . | Molecular weight, Da . | Radius of gyration, nm . | Hydrodynamic radius, nm . |
|---|---|---|---|
| No shear | 9.6 × 106 (± 3.7 × 106) | 97.1 (± 29.6) | 68.2 (± 19.2) |
| 2155/s for 30 s | 25.1 × 106 (± 6.9 × 106) | 113.8 (± 27.0) | 91.3 (± 22.9) |
| 6000/s for 120 s | 695.5 × 106 (± 152 × 106)* | 394.8 (± 117.7) | 466.4 (± 95.6)* |
| Shear applied . | Molecular weight, Da . | Radius of gyration, nm . | Hydrodynamic radius, nm . |
|---|---|---|---|
| No shear | 9.6 × 106 (± 3.7 × 106) | 97.1 (± 29.6) | 68.2 (± 19.2) |
| 2155/s for 30 s | 25.1 × 106 (± 6.9 × 106) | 113.8 (± 27.0) | 91.3 (± 22.9) |
| 6000/s for 120 s | 695.5 × 106 (± 152 × 106)* | 394.8 (± 117.7) | 466.4 (± 95.6)* |
Results are presented as mean (± SEM) for n = 3 VWF isolations.
Molecular weights and radii of gyration were obtained from SLS analysis and DLS analysis, respectively.
Significantly different from the unsheared sample.